益气补肾法抑制裸鼠乳腺癌骨转移的作用研究.pdfVIP

目录 中文摘要··························································I ABSTRACT ························································II 中英文缩略词······················································IV 前言······························································1 一、材料和方法····················································2 （一）、材料····················································2 （二）、方法····················································4 二、左心室注射法建立乳腺癌骨转移模型······························5 （一）、乳腺癌骨转移模型的复制和检测····························5 （二）、结果····················································8 三、益气补肾中药对于裸鼠乳腺癌骨转移的抑制作用····················9 （一）、模型构建和药物试验······································9 （二）、结果····················································11 四、讨论···························································12 结论······························································18 附图······························································19 参考文献··························································24 致谢·····························································27 文献综述：乳腺癌骨转移的中西医机制研究进展························28 综述参考文献······················································35 万方数据 中文摘要 益气补肾法抑制裸鼠乳腺癌骨转移的作用研究 目的：使用左心室注入法制造裸鼠乳腺癌骨转移模型，确定最佳的造模细胞浓度。 观察益气补肾中药对于裸鼠乳腺癌骨转移的抑制作用，为进一步明确作用机制做好前期 准备。 6 6 7 方法：1、将MDA-MB-231 细胞株培养后，采用 1×10 、5 ×10 、1×10 个/ml 三 种不同浓度的细胞悬液，使用左心室注射法，制造乳腺癌骨转移的动物模型，运用ECT 检查及病理检测的方法确诊骨转移，确定最佳的细胞浓度并观察造模效果。2 、运用左 心室注射法进行裸鼠乳腺癌骨转移造模后，将20 只裸鼠随机分成2 组，治疗组采用益 气补肾方药灌胃，对照组采用等量的去离子水灌胃治疗，共42 天，每3 天记录小鼠体 重，并根据体重调整药量。42 天后使用ECT 检查每只裸鼠的骨转移情况