不同核桃品种叶片和茎抗寒性测定.pdfVIP

山西农业科学2016 ， 44(7):945-950 Joumal of Shanxi Agricultural Sciences doi: 10.3969/j .issn.l002-2481.2016.07 .13 不同核桃晶种叶片和茎抗寒性测定 樊新萍，乔永胜，石美娟，田 鑫，王 勇，武彦霞 (山西省农业科学院果树研究所，山西太谷0308∞) 摘 要:为了探讨不同核桃品种及优系的抗寒机制，以核桃生长期叶片和休眠期一年生枝条为试材，应用石蜡切 片法、生理生化指标测定法，测定分析叶片结构、枝条生理生化指标与抗寒性之间的相关性。 SAS 统计分析结果表 明，栅栏组织厚度和叶片厚度、细胞结构紧密度(CTR 值)是影响抗寒性的主要组织结构指标，其叶片抗寒性强弱 排序为:金薄丰〉金薄香 1 号〉香玲〉西林2 号。在低温胁迫下，西扶 1 号、辽核 1 号、礼品 2 号当年生枝条的过 氧化物酶(POD)活性和可溶性蛋白含量比较高;而优系 FY-2、礼品 2 号、金薄香6 号等品种的脯氨酸(FAS)含量 和可溶性糖含量比较高。因此，核桃品种的抗寒性是植物适应环境的一个复杂的调控过程。 关键询:抗寒性测定;核桃;叶片组织结构;生理生化指标 中图分类号:S664. 1 文献标识码:A 文章编号:1∞2-2481(2016)07-0945-06 Determination of Cold Resistance on Leaves and Stems of DitTerent Walnut Cultivars FAN Xinping ,QIAO Yongshe吨，SHI Me加剧，TIAN Xin ,WANG Y ong ,WU Yanxia ( Instih血。f Pomology , Shan Abstract:To reveal mechanism of cold tolerance of different varieties of walnut and superior lines, walnut leaves and dormant annual branches were used to measurement of leaf structure, physiological and biochemical indicators and branches of the correlation between cold resistance by paraffin section method , physiological and bi∞hemical index determination method. Comprehensive index results showed 由at leaf thickness and palisade tissue thickness , cellular structure tightness (CTR) were the main factors related to 由e hardiness. The cold resistance for 4 cultivaJ百 was in order of Jinbaofeng Jinbaoxiang 1 Xiangling Xilin 2. Under low temperature stress, peroxidase (POD) activity and soluble protein content in Xiful , Liaohe 1 and Lipin 2 in annual branches were high. And proline and soluble sug缸 content in FY-2, Lipin 2 and Jinbaoxiang 6 were high. Therefore, the cold resistance of different walnut varieties is a complex regulatory process adapt to the environment. Key wor