10.1007_s10495-010-0537-1谢松强.pdfVIP

Apoptosis (2011) 16:27–34 DOI 10.1007/s10495-010-0537-1 ORIGINAL PAPER NPC-16, a novel naphthalimide–polyamine conjugate, induced apoptosis and autophagy in human hepatoma HepG2 cells and Bel-7402 cells Song-qiang Xie • Qian Li • Ya-hong Zhang • Jian-hong Wang • Zi-hou Mei • Jin Zhao • Chao-jie Wang Published online: 1 September 2010 Springer Science+Business Media, LLC 2010 Abstract The antitumor effects and molecular mecha- Keywords Apoptosis Autophagy Polyamine nism of NPC-16, a novel naphthalimide–polyamine con- conjugate Hepatoma carcinoma Caspase jugate, were evaluated in HepG2 cells and Bel-7402 cells. Apoptosis and necrosis were evaluated by Annexin V-FITC detection kit, and autophagy by acridine orange Introduction and Lyso-Tracker Red staining. The change of mitochon- drial transmembrane potential was measured using rhoda- Among the most devastating diseases, cancer annually mine 123 staining. The protein expression of Beclin 1, LC3 accounts for approximately 10 million new cases world- II and mTOR, p70S6 K, 14-3-3, caspase, and Bcl-2 family wide. The vast majority of anticancer drugs in clinical use members was detected by immunofluorescence assays and are limited by systemic host toxicity due to their non- Western Blot. Here, we elucidated the nature of cellular specific side effects [1]. Therefore, targeted therapies, response of HepG2 cells and Bel-7402 cells to NPC-16 at which are in contrast to the generalized cytotoxic effects of IC50. NPC-16 induced caspase-dependent apoptosis via the standard chemotherapy, may be a major breakthrough in mitochondrial pathway and death receptor pathway in the treatment of cancer. Bel-7402 cells. Differently, NPC-16 tr