菊芋生料联合生物加工发酵生产燃料乙醇.pdfVIP

安徽农业科学，Joumal of Anhui Agri. Sci.2012 ,40(9) :5438 -5441 责任编辑郑丹丹责任校对卢摇 菊芋生料联合生物加工发酵生产燃料乙醇 杨梅1 ，袁文杰2 ，风丽华3 (1 吉林化工学院环境与生物工程学院，吉林吉林 132022 ;2 大连理工大学生物科学与技术学院，辽宁大连 116023 ;3 中国石油吉林石化公司炼油厂，吉林吉林 132022) 摘要 [目的]在联合生物加工技术发酵菊芋生料生成乙醇工艺中，降低高浓度菊芋生料穆液的靠在度，使菊芋发酵乙醇浓度达到或超过 目前玉米乙醇的行业水平。[方法]利用马克斯克鲁维酵母(Kluyveromyces marxianus) ， 通过添加辅助酶制剂、改变拌料水温及优化补料 方式等方法，降低发酵穆液和度，提高菊芋生料的浓度，从而提高发酵终点的乙醇浓度并缩短发酵时间。[结果]最佳工艺条件为:45 c 水拌料，pH 5.0 ，添加 0.10% 酒精复合酶，发酵初始干粉浓度为 240 glL ，分别在发酵 12 和24h 补料，菊芋干粉终浓度可达到 3∞ glL，发 酵时间 48 h ，乙醇浓度达到 91. 6 岳IL， 乙醇对糖的得率为 0.464 ，为理论值的 90.6% 0 [结论]此工艺为菊芋乙醇工业化的生产提供了有 利条件。 关键词 菊芋;燃料乙醇;联合生物加工;马克斯克鲁维酵母;批式补料 中图分类号 S632.9 文献标识码 A 文章编号 0517 -6611 (2012)阴 -05438 -04 Production of Fuel Ethanol from Uncooked Helianthus ωberosus by Consolidated Bio-processing (CBP) Fermentation YANG Mei et aI (College of Environmental Biological Engineering , Jilin Institute of Chemical Technology , Jilin , Jilin 132022) Abstract [ Objective ] During the process of producing ethanol from uncooked Helianth旧 tuberosus by consolidated bio-processing (CBP) fermentation , the viscosity of high-concentration uncooked Helianthus tuberosus wort was decreased , which could reach or exceed tbe cu盯ent industtγlevel of com ethanol. [Method ] By using Kluyveromyces marxianus , through adding assistant enzyme , changing water temperature and opfimizing fed-batch metbod , the wort viscosity was decreased and concentration of uncooked Helianthus tuberosus was improved , and then , tbe final fermentation etbanol concentration was increased and fermentation time was reduced. [Result] The optimum processing condi- tions were: temperature of water 45 C, initial pH 5. 0 , adding 0.109奋 alcohol compound enzymes , fed-batched at 12 h and 24 h , initial con- centration of dry powder was 240 glL , the final concentration reached 300 glL , the final e