生物土壤结皮纤维素降解细菌分离鉴定及其降解特性分析.pdfVIP

内蒙古农业大学学报 第31 卷第4 期 Vo1. 31 No.4 2010 年 10 月 Jo山nal of Inner Mongolia Agricultural University Oct.201O 生物土壤结皮纤维素降解细菌分离、 鉴定及其降解特性分析. 李康宁， 丛立双， 王瑞刷， 冯福应\ 刘柯澜， 刘友来 (内蒙古农业大学生命科学学院应用与环搅徽生唱实验研究所，呼和浩特 01∞18) 摘要: 以竣甲基纤维素纳培养基为基础培养基，从内蒙古浑善达克沙地的生物土壤结皮中筛选、分离到 18 株具有 降解纤维素能力的菌株，采用刚果红培养基进行鉴定，依据水解圈大小并结合 DNS 法酶活测定结果选取了 4 株酶活 较高的菌株 HD1、HD2、HD3、和 HD4进行菌种鉴定。根据其菌落形态特征和 16SrDNA 序列分析结果，初步确定 HD1、HD2、HD3 属于芽袍杆菌属，HD4属于链霉菌属。其中以 HD1菌的纤维素酶活性最高，达到 14. 33U/此。 关键词: 浑善达克沙地; 生物土壤结皮; 分离鉴定; 纤维素降解菌; 纤维素酶活 中图分类号: 5154.3 文献标识码: A 文章编号:l(朋 -3575(2010)04 -0148-04 ISOLATION , LDENTIFICATION AND CHARACTERIZATION OF CELLULOSE - DEGRADING BACTERIA FORM BIOLOGICAL SOIL CRUSTS U Kang - ning , CONG Li - shuang , WANG Rui - g，缸tg ， FENG Fu - ying. , UU Ke -lan , UU Fa -lai (Institωefor applied Environmental Microbiology of College I!川ife Sciences Inner Mongolia Agricultural Univerity HuhJwt 01∞18) Abstract: C缸bo巧methyl cellul幽e sodium medium wereωed to isolate and screen cellul帽e-de刃，mp黯ing microorganis圃， and 18 strains were isolated from sample of biologicalω，il crust from D甸回 Hunshandak. Totally 18 s缸ains were isolated , 4 由ains (HD1 , HD2 , HD3and HD4) showed a great capacity in cellul曲edecomp黯i 吨， because large and distinct circles around theω，Ioniωin Congo red agar medium combined wÏth 由.eir enzyme activitiωwere observed by 也.e DNS method. on the basis of mo甲h口，logical characteristics of bacterial ∞，lonies and 16sDNA s呵uence 缸uúysis ， HD1, HD2, HD3 were identifiedωbeBacillωsp. While HD4 belongωStrepto­ myωs sp. HDl had the highest enzyme activity , which reached 14. 33U/ml. Key words: Desert h山回handak; biological