雾化吸入布地奈德对肺纤维化大鼠肺组织MMP2、TIMP2表达的影响论文.docVIP

　　雾化吸入布地奈德对肺纤维化大鼠肺组织MMP2、TIMP2表达的影响论文 张小波 ，范贤明，王文军，湛晓琴，曾鸣，朱晨 【摘要】 目的 探讨布地奈德雾化吸入对博莱霉素(BLM)致肺纤维化大鼠肺组织基质金属蛋白酶2（MMP2）及金属蛋白酶抑制剂2（TIMP2）表达的影响。方法 组)、生理盐水空白对照组(NS组)和布地奈德干预组（Bud组）各15只，BLM组和Bud组气管内灌注博莱霉素诱导肺纤维化.freelonary Fibrosis Zhang Xiaobo, Fan Xianming, ing, Zhu Chen, the Attached Hospital to Luzhou Medical College, Luzhou, Sichuan Province 646000 ABSTRACT Objective To investigate the effect of inhaled budesonide on the expression of matix metalloproteinases２（MMP２） and tissue inhibitors of metalloproteinases ２ (TIMP２) in lung tissues of rats ycin-induced pulmonary fibrosis. Methods 45 ) group, the budesonide (Bud) group and the normal solution (NS) group. Intratracheal perfusion of BLM ade to the Bud group and the BLM group to induce pulmonary fibrosis al solution to the NS group in the same condition. From 0 to 6 days after perfusion, aerosol inhalation of Bud al saline to other groups in the same condition. On the 7th, 14th, and 28th day,.freel each group each time, and the lung tissues ine the extent of alveolitis and pulmonary fibrosis, and then the expression levels of MMP２ and TIMP２ in lung tissues onary fibrosis of the Bud group uch higher than those of the NS group (Ｐ＜0.5) but much louch higher than those in the NS group (Ｐ＜0.5) uch loeliorate the extent of bleomycin-induced alveolitis and pulmonary fibrosis in lung tissues of the rats, the intervention mechanism is probably contributed to the reduction of the expression levels of MMP２ and TIMP２ in lung tissues and the regulation of the ratio of MMP２ and TIMP２ to some extent. KEYMP２） metalloproteinases ２ (TIMP２) 肺间质纤维化是许多慢性肺部疾病的终末病理过程，其发病率在全球呈上升趋势，其实质就是某些病理因素诱发肺损伤持续进行，继而导致细胞外基质（extracellular matrix ,ECM）代谢紊乱，某些细胞外基质成份在肺泡和间质内沉积，以及纤维组织过度修复造成肺组织结构紊乱。启动和调节肺损伤和持续过度修复的信号传导过程目前仍不清楚。近年来研究表明，基质金属蛋白酶（matrix metalloproteinases,MMPs）及其组织抑制剂（tissue inhibitors of metalloproteinases,TIMPs）在肺损伤和修复，以及肺纤维化的形成过程中起重要的调节作用。本实验采用博莱霉素(B1eomycin，BLM)对大鼠单次气管内灌注的方法，复制出大鼠肺纤维化模型，通过该模型了解布地奈德雾化吸入对肺组织中MMP2及TIMP2表达的影响，初