气相色谱-质谱联用法测定大枣低聚糖的单糖组成.pdfVIP

210 2009, Vol. 30, No. 16 食品科学 ※分析检测 1 1 2 林勤保 ，蒋梅峰 ，杨 春 (1. 山西大学应用化学研究所，山西太原 030006 ；2. 山西省农业科学院农产品综合利用研究所，山西太原 030031) 采用水浸提法提取、膜分离技术分离得到大枣粗低聚糖，再用Sephadex-G15 柱层析进行分离纯化，得到 大枣低聚糖，用气相色谱- 质谱联用法测定大枣低聚糖的单糖组成为阿拉伯糖、鼠李糖、核糖、甘露糖、半乳 糖和葡萄糖。同时测定了大枣中单糖的组成成分为果糖和葡萄糖。 大枣；低聚糖；气相色谱- 质谱联用；单糖组成 GC-MS Determination of Monosaccharide Composition of Oligosaccharides from Fruits of Chinese Jujube (Ziziphus jujuba Mill var. muzao) 1 1 2 LIN Qin-bao ，JIANG Mei-feng ，YANG Chun (1. Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China ； 2. Institute of Farm Products Comprehensive Utilization, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China) Abstract ：Oligosaccharides were produced from fruits of Chinese jujube by extraction using wa ter and membrane separation followed by purification by Sephadex-G15 column chromatography and were analyzed for their monosaccharide composition by GC-MS and monosaccharides obtained in the Sephadex-G15 column purification were also analyzed by GC-MS. The oligosaccharides were composed of arabinose, rhamnose, ribose, mannose, galactose and glucose. The monosaccharides from fruits of Chinese jujube were found to be mainly glucose and fructose. Key words ：Chinese jujube ；oligosaccharide ；GC-MS ；monosaccharide composition 中图分类号：TS207.3 文献标识码：A 文章编号：1002-6630(2009)16-0210-03 大枣是鼠李科(Rhamnaceae)枣属植物枣树(Ziziphus 1.1 材料与试剂 jujuba Mil1) 的果实，有极高的营养价值和很好的医疗保 大枣，采自山西临县，品种为山西木枣。 健作用。大枣中的糖分除了有大量的果糖和葡萄糖外， Sephadex-G15(100～200 目) 上海蓝