高氧液预处理对兔脊髓缺血损伤的保护作用及机制论文.docVIP

　　高氧液预处理对兔脊髓缺血损伤的保护作用及机制论文 桑韩飞，张英民，徐礼鲜，程虹，巩固，梅其炳，熊利泽 【关键词】 自由基 Protective effect of hyperoxic solution preconditioning on spinal cord ischemic injury in rabbits and its mechanism 【Abstract】 AIM: To investigate ic injury in rabbits and ale Nely assigned to 4 groups (8 in each group): Control group (C ), hyperoxic solution preconditioning (H), dimethylthiourea (DMTU, free radicals scavenger) + hyperoxic solution preconditioning group (D+H) and DMTU group (D). In H group, hyperoxic solution 10 ml kg-1 l kg-1 h-1 every day for 5 days; In the same inistred the same volume normal saline instead of hyperoxic solution; D+H group e as H group except for administering 500 mg kg-1 DMTU intravenously 1h before each hyperoxic solution preconditioning; D group inistered 500 mg kg-1 DMTU intravenously once a day for 5 days. 24 h after last preconditioning, the spinal cord ischemia in by infrarenal aortic occlusion. Neurologic status als oved for histopathologic study. RESULTS: The neurologic function scores and the number of normal neurons of anterior horn of spinal cord ber of normal neurons in anterior horn of spinal cord （r=0.804, P 0.01). CONCLUSION: Hyperoxic solutioin preconditioning has a significant protective effect on spinal cord ischemic injury in rabbits and oxygen free radicals may be involved in the protective effect. 【keyL kg-1高氧液.freelin匀速泵完，连续5 d；C组用同样方法给予等容量生理盐水；D+H组在每次高氧液预处理前1 h静脉给予500 mg kg-1 DMTU，其他处理同H组；D组每天静脉给予500 mg kg-1 DMTU，连续5 d. 最后一次预处理结束后24 h，夹闭所有动物腹主动脉肾下段20 min，制作兔脊髓缺血模型. 再灌注后48 h, 对所有动物神经功能评分， 然后处死动物取脊髓（L57），制作标本行组织病理学观察. 结果： H组的神经功能评分和脊髓前角正常神经细胞数明显多于C组、D+H组及D组(P 0.05)；C组、D+H组及D组的神经功能评分和脊髓前角正常神经细胞数组间无明显差异(P 0.05)；神经功能评分与其对应脊髓前角正常神经细胞计数之间有显著相关性（r=0.804,.freelethylthiourea, DMTU ）购于Fluka 公司. 高氧液用西安高氧医疗设备有限公司研制的“高氧医用液体治疗仪”制备，用量子化溶氧机制以生理盐水为载体，溶氧后可制成氧分压达到90～100 kPa及含微量臭氧的高氧液体. 1.2实验动物分组32只成年雄性新西兰大白兔，体质量2～2.5 kg，随机分成4组(n=8)，即对照组（C组）、高氧液预处理组（H组）、二甲基硫脲(DMTU, 自由基清除剂 ) +高氧液预处理组(D+H组)及DMTU组(D组). H组每天静脉给予10 mL kg-1高氧液，20 min匀速泵完，连续5 d；C组用同样方法给予等容量生理盐水；D+H组