Chapter 5.1 质谱概要1.pptxVIP

Chapter 6.1 Mass Spectrometry基本原理和仪器部分 ;Introduction;The elemental and isotopic composition of samples 样品的元素和同位素组成 Sequence determination of biomolecules 蛋白质和核酸测序分析 Coupling to separation techniques for organic and bioorganic analysis 复杂混合物成分的定量与定性分析 The structures elucidation of organic and biological molecules 有机化合物的结构解析 Characterization of chemical reaction 化学反应的表征 Mass spectral imaging 质谱成像 ;The block diagram shows the major components of mass spectrometers (MS). Sample inlet进样: introduce a very small amount of sample into the MS Ion sources离子源: convert the components of a sample into ions Mass analyzer质量分析器: separate ion based upon the m/z of the analyte ions Detector检测器: converts the beam of ions into an electrical signal that can then be processed, stored in the memory of a computer Data system: processes the data ;Basic principles of MS;Mass Spectrum质谱图;How mass spectra are communicated;Basic Terminology in Describing Mass Spectra基本术语;Total ion current (TIC) chromatogram;Base peak chromatogram;Extracted-ion chromatogram (XIC or EIC),;Mass scale 质量尺度 ;Isotope Peaks 同位素峰 ;在质谱图中得到的是同位素的组合;一些常见元素的同位素自然丰度; Calculation of atomic mass;相对质量 ;Monoisotopic Mass 单同位素质量;Role of the Electron Mass When Calculating Exact Mass;Accurate mass ;Number of Decimal Places When Calculating Exact Mass;Calculation of Isotopic Distributions;;;Performance of Mass Spectrometers;Signal-to-Noise Ratio describes the uncertainty of an intensity measurement. A quantitative measure of a signals quality by quantifying the ratio of the intensity of a signal relative to noise. Electronic noise, so-called “chemical” noise. A signal may be regarded to be clearly visible at S/N ≥ 10, a value often stated with detection limits. A mass spectrometer in good condition yields S/N 104 which means in turn that even isotopic peaks of low relative intensity can be reliably measured, provided there is no interference with background signals;Resolution and Resolving Power;Resolution;;Corre