山楂叶中总黄酮的含量测定方法研究.pdfVIP

江西中医学院学报20 07年1 2月第1 9卷第6期 JOURdWAL OFJIANGXI UNIVERSITYOF TCM2007 Vo1．19 No．6 山楂叶中总黄酮的含量测定方法研究* ★ 刘荣华1 邵峰 马志林 余伯阳 (1．江西中医学院现代中药制剂教育部重点实验室 南昌 330004；2．中国药科大学中药复方研究室 南京210038) 摘要：目的：研究山楂(山里红)叶中总黄酮的含量测定方法。方法：选择山里红叶中某一特定化学成分(牡荆素2 一 鼠李糖 苷)，比较该成分与总黄酮的含量关系，计算其比值，以该比值为换算因子，通过测定该成分的含量推算出山里红叶中总黄酮 的含量。结果：不同产地、不同采收期16个山里红叶样品中牡荆素2-0-鼠李糖苷与总黄酮的含量成显著正相关(r=0．950， P0．01)，且总黄酮与牡荆素2 一()_鼠李糖苷的含量比值(换算因子)为1、9493±0．2002。结论：可以将牡荆素2 一()_鼠李糖苷 的含量乘以换算因子求算出不同产地、不同采收期山里红叶中总黄酮的含量。 关键词：山楂；山里红；黄酮；药物分析 中图分类号：R 284．1 文献标识码：A Determination of Total Flavonoids in Leaves of Crataegus L． LIU Rong-hua ，SHAO Feng ，MA Zhi-lin ，YU B y 1．Key Laboratory ofModern Preparation of TCM，Jiangxi University of Traditional Chinese Medicine，Ministry ofEducation， Nanchang 330004 2．DepartmentofChineseComplexRecipeResearch，ChinaPharmaceutical University，Nanjing 210038 Abstract：Objective：Studying the method of dete~nination of total flavonoids in leaves of Crataegus pinnatifida gge．var．major N．E．Br．Method~Vitexin 2-O-rhanmoside was selected as the standard．The ratio of Vitexin 2-O-rhamnoside and the total flavonoids in leaves of Crataegus pinnatifida Bge．var．major N．E．Br．was calculated．The content of total flavonoids in leaves of Crataegus pinnatifida Bge．var．major N．E．Br．could be counted with the result of the content of the standard multiplied by the ratio．Results：The correlation of the content of Vitexin 2-O-rhanmoside and the total flavonoids in 16 kinds of leaves of Crataegus nnatifida Bge．var．major N．E．Br．from different locations and growth stages is significant，and the correlation coefficient WBS 0．950(P0．01，2-tailed)．The ratio of Vitexin 2-O-rhanmoside and the total flavonoids in leaves of Crataegus tn‘nnatifida Bge．Vrdr．major N．E．Br．was 1．9493±0．2002．Conclusion：The total flavonoids in leaves of Crataegus pinnatifida Bge．Var．major N．E．B