不同酸蚀时间处理后年轻及成年恒牙釉质表面微观形貌及性质变化的 .doc

不同酸蚀时间处理后年轻及成年恒牙釉质表面微观形貌及性质变化的观察研究 张 昊，马俊青，李 强，倪洁丽，倪媛媛，王 林* （南京医科大学口腔医学研究所，江苏 南京 210029） [摘 要]　目的：探讨酸蚀时间对年轻及成年恒牙釉质表面性质结构的影响。方法：收集因正畸需要而拔除的新鲜前磨牙90颗，其中年轻恒牙(患者年龄为12-17岁)45颗，成年恒牙(患者年龄≥18岁)45颗。扫描电镜下观察不同酸蚀时间处理后年轻恒牙与成年恒牙釉质表面微观形貌的差异，分析釉质表层元素含量、显微硬度，托槽抗剪切强度，ARI指数等性质的变化。结果：镜下形貌观察显示，年轻恒牙酸蚀15s后柱间质已开始出现溶解，酸蚀30s后釉柱宽度及高度均有降低，随着酸蚀时间的增加，釉柱间质溶解不断增多，釉柱正常结构逐渐被酸蚀破坏，酸蚀60s后釉质表面正常结构几乎消失；成年恒牙酸蚀30s后柱间质溶解逐渐明显，随酸蚀时间的延长，釉质表面形貌特点无明显差异。经检测，成年恒牙釉质表面的显微硬度值明显大于年轻恒牙。不同酸蚀时间处理后，年轻及成年恒牙托槽粘结后抗剪切强度均无显著差异，年轻恒牙钙磷含量和Ca/P比值无统计学差异，成年恒牙酸蚀60s后釉质表层磷含量明显小于45s，Ca/P比值小于30s、45s。结论：为了减少酸蚀对釉质表面的损害，年轻恒牙正畸临床酸蚀处理时间可适当减少至15s，成年恒牙可延长至30s。 [关键词] 年轻恒牙；成年恒牙；酸蚀时间；牙釉质 Characterization of the surface microtopography and properties of immature permanent and permanent dental enamel after variable etch times Zhang Hao, Ma Junqing, Li Qiang, Ni Jieli, Ni Yuanyuan, Wang Lin* （Institute of Stomatology, NJMU, Nanjing 210029, China） [Abstract] Objective: To investigate the effect of variable etch times on the characterization of immature permanent and permanent teeth. Methods: Collected 90 premolars extracted for orthodontic need, in which immature permanent teeth (patients aged 12 to 17) were 45 and permanent teeth (patients aged 18 or higher) were 45. Observed the differences of enamel surface microtopography between immature permanent and permanent teeth after variable etch times under scanning electron microscope (SEM), and also analysed the changes of enamel surface element content, microhardness, shear bond strength, index of ARI. Results: SEM showed that the interprismatic substance of immature permanent teeth has started to [基金项目] 国家自然科学基金项目2011-137）；江苏省研究生教育教学改革研究与实践课题（JGZZ13_050）；留学归国人员科研启动基金（2013-693） *通信作者（Corresponding author），E-mail：lw603@njmu.edu.cn dissolve after 15s. The width and height of prism had both decreased after 30s. With the increase of etch time, substance dissolved gradually and normal structure of enamel prism was destroyed by acid attack step by step. After 60s normal structure of the ena