枸杞多糖分离纯化及其结构光谱分析与显微学观察 - 天津中草药杂志社.pdf

中草药 Chinese Traditional and Herbal Drugs 第 43 卷 第 4 期 2012 年 4 月 • 645 • 枸杞多糖分离纯化及其结构光谱分析与显微学观察 * 郭 琦，孙润广 ，郭国，焦自明 陕西师范大学物理学与信息技术学院 生物物理与生物医学工程实验室，陕西 西安 710062 摘 要：目的 提取枸杞多糖并分析其结构性质和显微学形态，为枸杞的进一步开发利用提供参考。方法 采用水提醇沉法 得到枸杞多糖，Sephadex G-150 凝胶色谱柱纯化得 LBP3-I ，应用傅里叶变换光谱、气相色谱对 LBP3-I 进行组成结构分析， 通过环境扫描电镜和原子力显微镜观察该组分的显微学形态。结果 LBP3-I 是一种含有结合蛋白的多糖复合物。其单糖组 成为鼠李糖基、阿拉伯糖基、木糖基、甘露糖基、葡萄糖基、半乳糖基，且摩尔比率为 2.07 ∶2.38 ∶3.11 ∶1.00 ∶1.12 ∶2.86 。 红外光谱结果显示 LBP3-I 具有明显的糖类物质特征吸收峰且含有吡喃环；环境扫描电镜下 LBP3-I 呈现高分支结构，主要呈 链状，且糖链间多相互缠绕聚集成环状。结论 LBP3-I 是一种含有结合蛋白的多糖复合物，具有分支结构，主要成链状。 关键词：枸杞多糖；单糖组成；分支结构；环境扫描电镜；原子力显微镜 中图分类号：R284.18 文献标志码：A 文章编号：0253 - 2670(2012)04 - 0645 - 04 Isolation and purification, its structure spectral analysis, and microscopy observation of Lycium barbarum polysaccharide GUO Qi, SUN Run-guang, GUO Guo-yun, JIAO Zi-ming Laboratory of Biophysics and Biomedical Technology, College of Physics and Information Technology, Shaanxi Normal University, Xi’an 710062, China Abstract: Objective To provide the reference for further development and utilization of Lycium barbarums, the structure composition and morphology of L. barbarum polysaccharides (LBP) were studied. Methods LBP was extracted from L. barbarum with water, isolated and purified by quaternary ammonium salts precipitation and gel chromatography process. The structural composition of LBP3-I was characterized by FT-IR and gas chromatography (GC). The morphology was observed by environmental scanning electron microscopy (ESEM) and atomic force microscope (AFM). Results LBP3-I was a polysaccharide complex which contained protein. LBP3-I contained six kinds of monosaccharides (rhamnose, arabinose, xylose, mannose, glucose, and galactose), and their ratios were 2.07 ︰2.38 ︰3.11 ︰1.00 ︰1.12 ︰2.86. The FT-IR results revealed the typical characteristics o