热效应在微生物诱变育种中的应用.pdfVIP

微生物学通报 OCT 20, 2009, 36(10): 1592~1595 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 专论与综述 热效应在微生物诱变育种中的应用 高兴强 黄运红 戴 菲 付学琴 龙中儿* ( 330022) 摘 要: 本文综述了微生物诱变育种中的热效应机理及其应用研究进展。热在微生物诱变育种中 具有热诱变效应和热筛选效应, 热诱变效应通过热引起DNA 中G-C 碱基对的置换实现, 热筛选效 应可以从其他诱变剂诱变的菌体中获得更高的正向突变率。 关键词: 热诱变效应, 热筛选效应, 诱变育种 The Application of Heating Effect in Breeding of Microorganism * GAO Xing-Qiang HUANG Yun-Hong DAI Fei FU Xue-Qin LONG Zhong-Er (College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China) Abstract: Advances in mechanism and application of the heating effect in breeding of microorganism are reviewed in this paper. Heat produces mutagenesis effect and screening effect. Heating mutagenesis effect is occurred through the substitution of G-C base pair induced by heat, and heating screening effect produces higher forward mutation rate induced by other mutagens. Keywords: Heating mutagenesis effect, Heating screening effect, Mutagenesis breeding , , , 1 热诱变效应 , , 1.1 热诱变机理 , [3], , , , (EMSNTG ) (Escherichia coli)T4 [4−6] , , , [1]