毛细管等电聚焦电泳技术进展指南.pdfVIP

2003 3 V ol. 21 No. 2 M arch 2003 Chinese Journal of Chromatogr ap hy 121~ 125 杨 春, 姬 磊, 张维冰, 张玉奎 ( , 1160 11 : ( CI EF , , CI EF 59 : ; ; : O658 : A : 1000- 87 13( 200 3 02-0 12 1- 05 Advances i n Capillary Isoelectric Focusing Electrophoresis YA N G Chun, J I Lei, ZHAN G Weibing, ZH AN G Yukui ( National Chromatograp hic R . A . Center , Dalian Institute of Chemical Physics, The Chinese A cademy of Sciences, Dalian 11 011, China) Abstract: T he current st at us and latest advances in capillary isoelect ric focusing elect rophoresis ( CIEF are review ed from 59 recent research art icles. Evaluat ions and comments on carrier am- pholytes ( CA s , condit ioning or coat ings of capillary inner surface, and detect ion met hods are report- ed. In addit ion, the aspect s of qualitat ive analysis, peak capacit y, reproducibilit y in CIEF, and the fu- t ure applicat ions of CIEF in life science, are described . Key words: capillary isoelect ric focu sing elect rophoresis; advance; rev iew ( CIEF [ 1~ 6] 1 , CIEF ( CA s CIEF ( : [ 6] Hjert†n ( IEF A- , B- ( : , , , pH , pH , pI , pH , CAs[ 11, 12] , pH ( pH 3~ 10, 2 ( pI , , ~ 11 ( pH 6~ 8, 9~ 11 CA s , , IEF, , 280 nm CIEF IEF , 214 nm ,