嗜酸性乳杆菌培养上清液对实验性腹泻的作用.pdfVIP

· 药 物 与 临 床 · 嗜酸性乳杆菌培养上清液对实验性腹泻的作用 罗学娅，高卫，贾丛伟，齐丙迪，王纪人，李金红(大连大学医学院，辽宁省教育厅生物有机化学重点实验室，辽宁大连116622) 摘要：目的 研究嗜酸性乳杆菌LA14菌株耗尽培养上清液(spent ca[ture supernatant，SCS)对小鼠实验性腹泻的影响。方法 小鼠100只，随机分为10组 采用小鼠灌胃给予80 g·L 的番泻叶水煎剂(25 mL-kg )或蓖麻油(15 mL- 。)，造成 小鼠实验性腹泻动物模型，造模前分别以SCS、活菌、SCS+活菌25 mL- ·次 给小鼠灌胃，以及思密达冲剂3 g· 。。· 次 给小鼠灌胃，每天2次，连续3 d。造模后连续观察6 h内小鼠腹泻累积次数、腹泻发生率和稀便率。结果 在SCS组、活 菌组以及SCS+活菌组，两种实验性腹泻小鼠的稀便率均有降低，与模型组比较差异显著(P0。05或P0。01)，同时各时间 点腹泻累积次数也有所下降，与模型组比较差异显著(P0。05或P0．O1)。结论 嗜酸性乳杆菌SCS和活菌均有一定的抗 小鼠实验性腹泻的作用 关键词：嗜酸性乳杆菌；耗尽培养上清液；腹泻 中图分类号：R969 文献标识码：A 文章编号：1001—2494(2007)24—1908—03 Effects of Spent Culture Supernatant of Lactobacillus Acidophilus on Experimental Diarrhea LUO Xue—ya，GAO Wei，3IA Cong—wei，QI Bing—di，WANG Ji—ren，LI Jin—hong(Medical Coll~ge of Dalian University，Bio organic Chemistry Key Laboratory 船Education Department ofLiaoaing Provim：e，Dalian 116622，China) ABSTRACT：OBJECTIVE To study the effects of the spent culture supernatant(SCS)of Lactobacillus acidophilus strain LA14 on experimental diarrhea in mice METHODS 100 Mice were randomly divided into 10 groups．The animal model of the experimental di— arrhea was made by intraperitoneal administration of 80 g·L decoction of Cassia angustifolla leaf(25 mI ·kg )or castor oil(15 mL·kg )to mice．Before the model was made，SCS，living bacteria，SCS plus living bacteria(25 mI ·kg times)and Smecta (3 g·kg times)were respectively administered(twice per day)to mice for three days．After the model was made，the accumulated times of diarrhea．the ratio of diarrhea and the ratio of loosing stool of mice were measured within six hours．1IESULTS In SCS group． 1iving bacteria group and SCS plus living bacteria group，the ratios of loosing stool in mice with two kinds of experimental diarrhea were reduced．Compared with model group，the difference was significant(P0．05 or P0．01)．Meanwhile the accumulated times of diar— rhea in mice of different time points were also dec