外源ABA、BR和ETH对番茄果实番茄红素含量的影响.pdfVIP

沈阳农业大学学报，2007—12，38(6)：784—787 Journal of Shenyang Agricultural University，2007—1 2，38(6)：784—787 外源ABA、BR和ETH对番茄果实 番茄红素含量的影响 翁 倩。，周宝利 ，于 洋 ，付亚文a (沈阳农业大学 a．园艺学院，b．生物科学技术学院，沈阳 110161) 摘要：为探索外源激素对番茄果实中番茄红素含量的影响，改进从番茄果实中正己烷、乙醇以及丁烯羟基甲苯BHT的丙酮混合试 剂提取番茄红素的方法，确定了番茄红素最佳提取条件，并利用该条件检测分析了外源ABA(脱落酸)、BR(油菜素内酯)和ETH(~ 烯)处理番茄果实后番茄红素的含量。结果表明：200mg-L- 的外源ABA、0．001mg-L- 的外源BR和3000mg．L-l的外源ETH对促进 番茄果实中番茄红素含量增加的效果较好，番茄红素含量分别达到1l5．1llxg·g- ，106．16Ixg-g_‘FⅣ和125．21txgg- 。外源ABA 、 BR和ETH均能在不同程度上促进番茄果实中番茄红素含量增加。 关键词：番茄(Lycopersicon esculentum Mil1．)；番茄红素；ABA；BR；ETH 中图分类号：$641．2 文献标识码：A 文章编号：1000—1700(2007)06—0784—04 Effects of Exogenous ABA,BR ETH on Changes of Lycopene’S Contents in Fruit of Tomato WENG Qian ，ZHOU Bao-li ，YU Yangb，FU Ya—wen a．College of Horticulture，b．College of Biological Science and Technology，Shenyang Agricultural University，Shenyang 1 10161．China) Abstract：The obj ective of experiment is the effect of lycopene content in ripe fruit of tomato after exogenous phytohormone treatments at the green mature stage of ripening．A method for extracting lycopene in tomato by mixing hexane，ethanol and acetone of butylated hydroxytoluene(BHT)was improved．As a result，the optimum condition was obtained．With the optimum condition，the contents of lycopene in ripe fruit after exogenous ABA，BR and ETH treatments at the green mature stage of ripening were determined．Results showed that 200 mg·L～ of ABA．0．001mg-L～ of BR and 3000mg-L一 of ETH had better ef- feets on the accumulation of lycopene in fruit of tomato．The contents of lycopene in fruit of tomato reached 1 15．1 1 Ixg·g-lFW ， 106．16)xg·g-iFW and 125．211xg·g-lFW respectively．Exogenous ABA，BR and ETH accelerated the accumul