微乳液相色谱法测定他汀类药物.pdfVIP

Vo l. 40 No.2 浙江工业大学学报 第 40 卷第 2 期 2012 年 4 月 JOURNAL OF ZHEJIANG UNIVERSITY OF TECHNOLOGY Apr. 2012 微乳液相色谱法测定他汀类药物 施介华l.2 ，薛竹1 (1.浙江工业大学药学院，浙江杭州 310032 ， 2. 浙江工业大学绿色合成技术国家重点实验专培育基地，浙江杭州 310032) 摘要:以微乳液相色谱法分离测定普伐他汀锅、阿托伐他汀钙、辛伐他汀和洛伐他汀.考察了微乳液 组成表面活性剂质量分数、油相质量分数、助表面活性剂质量分数以及 pH 值等对他iT 类药物分离 测定的影响，确定最佳的色谱条件.实验结果表明:等梯皮微乳液相色谱具有较强的同时快速分离 疏水性差异较大的混合物的能力.该方法具有操作简便、快速，准确性和重现性好等特点.对四种制 剂供试品多次测定，普伐他汀锅、阿托伐他汀钙、辛伐他汀和洛伐他汀的相对标准偏差(RSD) 分别 为 0.39% , 0. 45% ， 0.053% 和 0.25%. 加入回收率分别为 97.8%-101. 2% , 10 1. 5%-102.7% , 97.7%-10 1. 9%和 98.7%-101. 9%. 关键词:微乳液相色谱z 普伐他汀纳;阿托伐他，汀钙;辛伐他汀z 洛伐他汀;含量测定 中图分类号:0657.7 文献标志码:A 文章编号: 1006-4303(2012)02-0119-05 Separation and determination of statins by microemulsion liquid chromatography SHI Jie-hual •2 , XUE Zhu l (1. College of Pharmaceutical Science. Hangzhou 310032. China; 2. State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology. Zhejiang University of Technology. Hangzhou 310032. China) Abstract: Pravastatin sodium , atorvastatin calcium , lovastatin and simvastatin were determined by microemulsion liquid chromatography. The effect of concentration of surfactant , cosurfactant and lipophilic organic solvent and pH value on determination and separation efficiencies of statins had been investigated to get the best chromatography conditions. The experimental results showed that isogradic microemulsioÐ liquid chromatography posses better ability in rapid separation of statins mixture whic