FLP_FRT位点特异性重组系统在高等真核生物中研究进展.pdf

中国农业科学 2011,44(15):3252-3263 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2011.15.021 FLP/FRT 位点特异性重组系统在高等真核生物中的研究进展 赵爱春，龙定沛，谭 兵，许龙霞，向仲怀 （西南大学蚕学与系统生物学研究所/农业部蚕学重点开放实验室，重庆 400716 ） 摘要：来源于酵母2 μm质粒的FLP/FRT 位点特异性重组系统已经被广泛应用于拟南芥、水稻、小鼠、果蝇、 线虫等高等真核模式生物，正逐渐成为转基因动植物研究领域进行基因操作的强有力工具。笔者综述了 FLP/FRT 系统的重组原理及其在高等真核生物中的应用，系统地概述了该系统在转基因植物、哺乳动物、昆虫以及其它相 关高等真核模式生物中的研究现状，讨论了FLP/FRT 系统在研究中存在的主要问题、应用前景和发展方向。 关键词：FLP/FRT 系统；位点特异性重组；转基因；高等真核生物 Progress in Research of FLP/FRT Site-Specific Recombination System in Higher Eukaryotes ZHAO Ai-chun, LONG Ding-pei, TAN Bing, XU Long-xia, XIANG Zhong-huai (Institute of Sericulture and Systems Biology, Southwest University/Key Sericultural Laboratory of the Ministry of Agriculture, Chongqing 400716) Abstract: FLP/FRT site-specific recombination system derived from 2 μm plasmid of yeast has been widely used in Arabidopsis thaliana , Oryza sativa Linnaeus, Mus musculus , Drosophila melanogaster , Caenorhabditis elegans and other higher eukaryotic organisms, and gradually become one of the powerful tools of genetic manipulation in transgenic animals and plants research areas. This review introduced the recombination principles of FLP/FRT system and its application in higher eukaryotes, and systematically summarized the main achievements of the system in transgenic plants, mammals, insects and other higher eukaryotic model organisms. In addition, the main problem, application prospect and developmental trend of the FLP/FRT system were discussed in this review. Key words: FLP/FRT system; site-specific recombination; transgene; higher eukaryotes