基于RNA测序技术的转录组从头拼接算法研究概要1.docVIP

基于RNA测序技术的转录组从头拼接算法研究 学院： 专业： 班级： 学生姓名： 学号： 指导老师: 完成日期： Abstract: The main field of bioinformatics research in molecular biology, and for the field of molecular biology, scratch spliced transcripts group is the core content, namely the use of transcriptase sequencing fragments of mosaic in the entire transcriptase expression of all transcripts. The emergence of RNA sequencing, spliced transcripts in the calculation to provide a certain set of challenges. At the moment, stitching algorithm transcriptase mainly splicing method reference genome from scratch stitching method. Although the reference genome-based approach is more than de novo mosaic method breakthrough, but based on the reference genome splicing method, there are still some fatal flaw, that is to have a high-quality reference genome. From the analysis of the actual situation, the vast majority of organisms does not exist an alternative known reference genome, by contrast, the importance of the first stitching algorithm to highlight out. Based on this phenomenon, this paper based on the analysis of the current stitching algorithm, proposed a new de novo transcriptase stitching algorithm (Bridger), clever use of some techniques based on the reference genome de novo algorithm to supplement the current stitching algorithm. With people, test results RNA sequencing data on dogs and rats, to show all current Bridger prominent than stitching algorithm from scratch. In addition, by way of example will demonstrate the important value of Bridger in practical applications. Finally, summarize, and further describes some research work and research transcriptase downstream splicing. Keywords: bioinformatics; reference genome; stitching algorithm; sequencing data 目 录 摘要…………………………………………………………………………………………………..ii 第一章 绪论 第一章 分子生物学的背景知识 1.1生物学基础…………………………………………………………3 1.2测序技术3 1.2.1测序技术的发展…………………………………………………………………………3 1.2.2单端测序和双端测序……………………………………………4 1.2.3 RNA 测序………………………………………………………………………………….5 1.3 测序片段的拼接………………………………………