pEF1 myc-His B使用说明.pdf

pEF1/myc-His B 编号 载体名称 北京华越洋VECT76095 pEF1/myc-His B pEF1/myc-His B载体基本信息： 别名: pEF1/myc-HisB 质粒类型: 哺乳动物表达载体 表达水平: 高拷贝 启动子: EF-1a 载体大小: 6169 bp 5 测序引物及序列: T7 Fwd：5d[TAATACGACTCACTATAGGG]3 载体标签: 6X His, myc 载体抗性: Ampicillin (氨苄青霉素) 筛选标记: Neomycin pEF1/myc-His B载体图谱: pEF1/myc-His B载体简介： pEF1/myc-His A, B, and C are 6.2 kbvectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells.The vectors containthe following elements: 1. Human elongation factor 1?-subunit (hEF-1 α) promoter for high-level expression across a broad range of species and cell types (Goldman et al., 1996; Mizushima and Nagata, 1990). 2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc (c-myc) epitope and a polyhistidine metal-binding peptide. 3. Neomycin resistance gene for selection of stable cell lines. 4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g., COS7). The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive controlfor transfection, expression, and detection inthe cell line of choice. pEF1/myc-His B载体序列： AATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAA TAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTC CCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCC TGCTTGTG