大花蕙兰原球茎一步成苗快速繁殖研究.pdfVIP

安徽农业科学，Journal ofAnhui Agri．Sci．2007，35(35)：11392—11393 责任编辑 张杨林 责任校对 王森 大花蕙兰原球茎一步成苗快速繁殖研究 魏琴1，曾J顿洪1，王丽2，余庆华3(1．宜宾学院西南特色经济植物保护与利用重点实验室，四川宜宾644o07；2．四川农业大学，四川雅 安 625014；3．四川省凉山州教科所，四川西昌 615000) 摘要 f目的】筛选适宜原球茎一步成苗的培养基，探讨原球茎试管苗的快繁移栽技术。『方法1以大花蕙~(CymbidiMm and orum)幼 嫩芽为外植体进行组织培养研究。以Ms为基本培养基附加不同的激素，分别设计5种配方的原球茎诱导培养基和3种配方的原球 茎一步成苗培养基，原球茎一步成苗的最适培养基。用农用链霉素稀释2 500～3 500倍对原球茎试管进行消毒后，栽入不同的基质， 研究原球茎试管苗的移栽技术。f结果1大花惠兰在MS+6-BA 1．0 mg／L+NAA 0．1 mg／L配方培养基上诱导原球茎效果最好。试管苗的 快繁以MS+6_BA 2．0 mg／L+NAA 0．2 m 的培养基为最适，2l d后原球茎大量增殖和分化，诱导出丛生芽并生根。在腐殖土中适当使 用农用链霉素有利于练苗成功。『结论1该试验探究出原球茎从增殖，诱导分化到生根只用一种培养基，大大缩短生产周期，但其生根 率只有75％，需做进一步研究。 关键词 大花蕙兰；一步成苗；原球茎 中图分类号 Q943．1 文献标识码 A 文章编号 05l7—66ll(2007)35一l1392—02 Study on One-step-seedling Formation and Rip Reproduction in Protocorm of Cymbidium grandiflorum WEI qin et al (Key Laboratory of Protection and Utilization to Southwestern Characteristic Economics-plant，Yibin University，Yinbin，Sichuan 6440H0021 Abstract 『Obiective1 The aim of the research was to screen medium suitabIe for one_step-seedling formation and discuss transplanting technique．[Method】In the tissue culture Cymbidium grandiflorum，with the tender bud of C、grandiflorum as explant，Ms as the basic medium was added with the different hormones，5 formulas of protocorm induction media and 3 formulas of protocorm one-step-seedling formation media were designed to screen the optimum medium suitable f0r one．step．seedling formation．The tub seedlings of protocorm were sterilized with agricultural streptomycin diluted at 2 300-3 500 times，then transplanted on the different matrixes to study the transplanting technique of protocorm tub seedlings．[ResultJ The formula medium of MS+6一BA 1．0mg／L+NAA 0．1mg／L got best induction effect on for protocorm induction of C grandiflorum．The formula medium of MS+6一BA 2．0 mg／L+NAA 0．2 mg／L was most suitable to the rip reproduction of protocorm tub seedlings． After 3 weeks o