微量测序新方法2015.pptVIP

Direct Sequencing from the Minimal Number of DNA Molecules Needed to Fill a 454 Picotiterplate PLOS ONE 2014 454 sequencing platforms: Starting material for a rapid library in 454 FLX + technology: 1ug However, quite often the amount of DNA available is limited: biopsies, laser dissection experiments, genomics for non-cultivable microorganisms, single cell genomic experiments, etc. The most commonly used method to increase the initial amount of DNA for sequencing: Multiple Displacement Amplification (MDA) MDA Bias: Genome coverage bias. In addition, regions of high GC content could lead