正相高效液相色谱法确证土壤中氯氰菊酯的残留量.pdfVIP

第28卷 第6期 青 岛科 技 大 学 学报(自然科学版) Vo1．28 No．6 2007年12月 Journal of Qingdao University of Science and Technology(Natura1 Science Edition) Dec．2007 文章编号：1672—6987(2007)06—0498—04 正相高效液相色谱法确证土壤中 氯氰菊酯的残留量 范志先，沈翠丽，赵文英，丁 宁 (青岛科技大学 化工学院，山东青岛266042) 摘 要：采用正相高效液相色谱(NPIHPLC)和气相色谱一电子捕获检测器(GC—ECD)测定 了土壤中氯氰菊酯的残留量。NPIHPLC法的检出限为 3×10 g，定量限为 0．03 mg·kg-。。土壤中氯氰菊酯的定量确证试验结果表明，对2个土壤田间试验样品， 使用 NP．HPLC方法测得的数据平均值分别为 0．295和 0．498 mg·kg_。，RSD分别为 2．8 和 1．0％；使用 GCIECD方法测得的数据平均值分别为 0．308和 0．501 mg· kg_。，RSD分别为7．7 和3．6 。2种方法的准确度和精密度均符合农药残留试验准 则的要求，且可互相证明测定的数据准确可靠。NP—HPLC测定数据与GC—ECD测定数 据相比，RSD较小。 关键词：氯氰菊酯；土壤；农药残留；NP—HPI C；定量确证 中图分类号：TQ 453．21 文献标识码：A Confirmation and Determination of Cypermethrin Residue in Soil by NP—HPLC FAN Zhi。xian，SHEN Cui-li，ZHAO Wen-ying，DING Ning (College of Chemical Engineering，Qingdao University of Science and Technology，Qingdao 266042，China) Abstract：The determination of cypermethrin residue in soil was studied by NP-HPLC method using hexane and tetrahydrofuran of 99：1( ／ )as mobile phase．The detec— tion limit was 3×10 g，and the quantification limit was 0．03 mg·kg ．The results of quantitative confirmation for two soil samples showed that the average values were 0．295 mg·kg and 0．498 mg·kg～ ，and the RSDs were 2．8 and 1．0 respectively by NP-HPLC，while the average values were 0．308 mg·kg～ and 0．501 mg·kg_。， and the RSDs were 7．7 and 3．6 respectively by GC-ECD．The accuracy and preci— sion of the both methods could accord with Guideline on pesticide residue trials in PR China．Compared with GC—ECD，the RSD of the data determined by NP—HPLC was smaller． Key words：cypermethrin；soi