EF24对重症急性胰腺炎大鼠肠道损伤的影响.pdfVIP

目 录 中文摘要······································································1 英文摘要···········································································4 研究论文 EF24 对重症急性胰腺炎大鼠肠道损伤的影响 前言···········································································8 材料与方法···································································9 结果·········································································21 附图·······································································24 附表·········································································31 讨论·····································································33 结论··············································································36 参考文献·····························································37 综述 急性胰腺炎治疗的实验性研究·········································39 致谢·····················································································48 个人简历·····································································49 万方数据 中 文 摘 要 EF24 对重症急性胰腺炎大鼠肠道损伤的影响 摘 要 重症急性胰腺炎(severe acute pancreatitis，SAP)是多种原因引起胰酶 激活导致胰腺组织水肿、坏死和出血，以致全身脏器功能衰竭的炎症性疾 病。虽然内镜括约肌切开术治疗胆结石患者，选择性肠道净化和预防性抗 生素的使用可能对治疗胰腺炎是有益的，但是一直没有特效的治疗药物。 肠道屏障功能在 SAP 的恢复中发挥着重要的作用，所以研究影响肠道屏 障的因素，减少SAP 的肠道损伤，有助于SAP 的治疗。近年来姜黄素对 SAP 的作用越来越受到大家的关注。 姜黄素是多酚类化合物，具有抗氧化性、抗炎症反应、抗病毒、抗肿 瘤等作用。姜黄素可以引起生长因子、蛋白激酶及转录因子表达的变化。 联苯二氟酮EF24 是姜黄素的类似物，目前研究EF24 主要围绕在促进肿 瘤细胞凋亡及发现治疗肿瘤的方法上，尚无EF24 与急性胰腺炎及肠粘膜 屏障方面的研究报道。Vivek R. Yadav 等证明EF24 能抑制失血性休克时 的炎症反应，因而本研究从抗炎症反应的角度分析姜黄素类似物EF24 对 急性胰腺炎肠粘膜屏障的作用。 目的：本实验旨在通过观察SAP 时胰腺、肠道组织学的变化，检测 肠道组织p65 、Claudin-1 蛋白和mRNA 的表达情况和血清IL-6、TNF-α 的浓度，探索联苯二氟酮