多重PCR方法检测艰难梭菌毒素A基因(tcdA)、毒素B基因(tcdB)和二元毒素基因(cdtAcdtB).pdfVIP

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多重PCR方法检测艰难梭菌毒素A基因(tcdA)、毒素B基因(tcdB)和二元毒素基因(cdtAcdtB).pdf

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多重PCR方法检测艰难梭菌毒素A基因(tcdA)、毒素B基因(tcdB)和二元毒素基因(cdtAcdtB)

目录缩略词表 „„„„„„„„„„„„„„„„„„„„„„„„„„„„ 3 中文摘要 „„„„„„„„„„„„„„„„„„„„„„„„„„„„ 4 Abstract „„„„„„„„„„„„„„„„„„„„„„„„„„„ 5 前言 „„„„„„„„„„„„„„„„„„„„„„„„„„„ 6 材料与方法 „„„„„„„„„„„„„„„„„„„„„„„„„ 8 结果 „„„„„„„„„„„„„„„„„„„„„„„„„„„„ 14 讨论 „„„„„„„„„„„„„„„„„„„„„„„„„„„„„ 18 全文结论 „„„„„„„„„„„„„„„„„„„„„„„„„„„ 22 参考文献 „„„„„„„„„„„„„„„„„„„„„„„„„„„ 23 致谢 „„„„„„„„„„„„„„„„„„„„„„„„„„„„„ 27 综述：艰难梭菌感染的研究进展 „„„„„„„„„„„„„„„„„ 28 2 缩略词表缩略词英文全称中文全称 AAD Antibiotic Associated Diarrhea 抗菌药物相关性腹泻 CCFA Cycloserine-Cefoxitin-Fructose Agar 环丝氨酸-头孢西丁- 果糖琼脂 CD Clostridium difficile 艰难梭菌 CDI Clostridium difficile Infection 艰难梭菌感染 CDT Binary Toxin 二元毒素 cdtA/cdtB CDT gene A/ CDT gene B 二元毒素基因 DNA Deoxyribonucleic Acid 脱氧核糖核酸 EB Ethidium Bromide 溴化乙锭 EIA Enzyme Immunoassay 酶免疫测试 ELFA Enzyme-Linked Fluorescent Assay 酶联荧光测定 ELISA Enzyme-Linked Immunosorbent Assay 酶联免疫吸附试验 EP Eppendorf Tube 微量离心管 GDH Glutamate Dehydrogenase 谷氨酸脱氢酶 PaLoc Pathogenicity locus 致病决定区 PCR Polymerase Chain Reaction 聚合酶链反应 PFGE Pulsed Field Gel Electrophoresis 脉冲场凝胶电泳 PMC Pseudo-Membrane Colitis 伪膜性肠炎 TcdA Toxin A of Clostridium difficile 艰难梭菌毒素A tcdA Toxin A gene 毒素A 基因 TcdB Toxin B of Clostridium difficile 艰难梭菌毒素B tcdB Toxin