中心组合设计-响应面法优选酸枣仁总皂苷的提取工艺研究.pdf

5 11 Vol. 5 No. 11 2014 11 Journal of Food Safety and Quality Nov. , 2014 - 祝洪艳, 林海成, 杨 鹤, 王国丽, 赵 岩, 郜玉钢, 张连学* (, 130118) 摘 要: 目的 方法 , -, SAS , 结果 14 75%2 , 2 h 34.26 mg/g结论 - , , , , 关键词: ; A; ; - Optimization of reflux extraction of jujuboside from semen ziziphi spinosae by central composite design-response surface methodology ZHU Hong-Yan, LIN Hai-Cheng, YANG He, WANG Guo-Li, ZHAO Yan, GAO Yu-Gang, * ZHANG Lian-Xue (College of Chinese Medicinal Materials, Jilin Agricultural University , Changchun 130118, China) ABSTRACT: Objective The extraction of jujuboside from semen ziziphi spinosae was optimized. Methods The content of jujuboside was detected by ultraviolet and visible spectrophotometry, and central composite de- sign (CCD)-response surface methodology was used to study the effects of ethanol concentration, ethanol do- sage and extraction time as well as their interactions on the content of jujuboside. A predictive model was de- veloped by SAS software, and the optimum extraction conditions were determined by response surface analysis. Results The optimum reflux extraction conditions were 14 times of 75% ethanol extracted 2 h for 2 times. The average content of jujuboside was 34.26 mg/g. Conclusion CCD-response surface methodology for op- timizing the reflux extraction press of juj