基于pJFH-1的HCV全基因组扩增方法的建立-第三军医大学学报.docVIP

基于pJFH-1的HCV全基因组扩增方法的建立 郭艳、兰林、何长龙、洪国祜、程玲、毛青（40038 重庆，第三军医大学西南医院研究Amplification of full-length hepatitis C virus genome based on pJFH-1 Guo Yan, Lan Lin, He Changlong, Hong Guohu, Cheng Ling, Mao Qing (Department of infectious diseases, Southwest Hospital, Third Military Medical University, Chongqing 400038, China) [Abstract] Objective To optimize the protocol of long-PCR for amplifying full-length HCV genome based on pJFH-1. Methods Optimization of long PCR strategies by testing a series of primers, adding various concentrations of glycerol, DMSO or both, using different cycle systems, then s