高效离子交换色谱法测定半乳糖、葡萄糖、乳糖及低聚半-食品科学.PDFVIP

180 2014, Vol.35, No.06 食品科学 ※分析检测 高效离子交换色谱法测定半乳糖、葡萄糖、 乳糖及低聚半乳糖含量 1 1,* 1 2 郑惠玲 ，邓宝浣 ，肖桂秋，钟新林 （1.量子高科 （中国）生物股份有限公司，广东 江门 529081； 2.赛默飞世尔科技应用研究中心广州实验室，广东 广州 510070 ） 摘  要： β 建立离子色谱法定量测定以乳糖为原料、使用 -半乳糖苷酶生产的低聚半乳糖产品的方法。样品前处理过 程中用体积稀释法代替质量稀释法，采用高效CarboPac PA20 阴离子交换色谱柱和积分安培法检测，并对淋洗液组 成进行优化。优化后的方法加标回收率90.58%～99.45%，相对标准偏差为3.54%。半乳糖、葡萄糖、乳糖的方法检 出限分别为0.24 、0.59 、0.75 μg/g 。半乳糖和葡萄糖在0.2～7 μg/mL 、乳糖在0.6～10 μg/mL 的范围内，其质量浓度 与峰面积线性关系良好，相关系数在0.999 2～0.999 9之间。用此方法测定6 种原料低聚半乳糖，目标组分总含量为 95.84%～10 1.19%。 关键词：高效离子交换色谱；低聚半乳糖；积分安培 Determination of Galactose, Glucose, Lactose and Galacto-Oligosaccharide by High Performance Ion Exchange Chromatography 1 1,* 1 2 ZHENG Hui-ling , DENG Bao-huan , XIAO Gui-qiu , ZHONG Xin-lin (1. Quantum Hi-Tech Biological Co. Ltd., Jiangmen 529081, China; 2. Guangzhou Laboratory of Application and Research Centre, Thermofisher Scientific, Guangzhou 510070, China) Abstract: A method was established to determine galacto-oligosaccharide (GOS), synthesized with β -galactosidase using lactose as the precursor, by ion exchange chromatography. The pre-treatment of samples was achieved through volume dilution instead of gravimetric dilution. CarboPac PA20 anion exchange column and pulsed amperometric detector were used to determine the contents of GOS. The recovery of the proposed method was 90.58%–99.45% and the relative standard deviation was 3.54%. The detection limits for gluctose, galactose and lacto