水稻早衰叶突变体es-t的遗传分析及精细定位.docVIP

水稻早衰叶突变体es-t的遗传分析与精细定位 本文档由【中文word文档库】提供，转载分发敬请保留本信息； 中文word文档库免费提供海量范文、教育、学习、政策、报告和经济类word文档。 杨窑龙1)* 饶玉春1)* 刘慧娟1, 2) 钱前1)? 曾大力1)? （1. 中国水稻研究所水稻生物学国家重点实验室, 杭州, 310006；2. 杭州师范大学，杭州，310036） *同等贡献 qianqian188@; dalizeng@126.com 摘要：突变体es-t是EMS诱变处理日本晴后获得该突变体主要为叶片从苗期开始，随着其生长发育发黄叶片伴有铁锈色的小斑点，叶尖和叶缘为，严重的早衰es-t (early senescence-temporary)。es-t为新发现的早衰突变体定位42.1kb的物理区间内，为进一步克隆该基因阐明叶片早衰的分子机制奠定基础。关键词：水稻；早衰突变体es-t；基因定位Yang Yaolong1)* Rao Yuchun1)* Liu Huijuan1,2) Fang Yunxia1) Dong Guojun1) Huang Lichao1) Leng Yujia1) Guo Longbiao1) Zhang Guangheng1) Hu Jiang1) Gao Zhenyu1) Qian Qian1)? Zeng Dali1)?(Z1.China National Rice Research Institute, Hangzhou China, 310006; 2.Hangzhou Nprmal University, Hangzhou China, 310036) * These authors contributed equally to this work. ? To whom correspondence should be addressed. E-mail: qianqian188@, dalizeng@126.com Abstract: An es-t (early senescence-temporary ) mutant derived from the EMS treatment of Nipponebare was identified in rice. Its leaf shows yellow since seeding stage, and the chlorophyll content is also decreased in es-t. The rusty spots were found with its growth, especially in leaf margin and tip. The plant show a typical early-senescence in milky stage. es-t shows smoother than wild type in leaf surface under scanning electron microscope, it lacks siliceous protuberance around the stoma in leaf. The chloroplast grew abnormally and filled with many starch grains in es-t. Paraffin section analysis shows the development of prothenchyma and vascular bundle was also abnormal in the mutant. Genetic analysis indicated that es-t was controlled by a recessive gene, which was finally fine delimited in a 42-kb interval on chromosome 5. Key words: Rice; early senescence mutant; gene clone 叶片是植物进行光合作用和呼吸作用的主要器官，对植物体的生命活动起着重要的作用。叶片的发育植物形态建成的重要，与植物株型和农业产量的形成密切相关[12]。水稻叶片衰老可导致光合能力的降低和同化能力的逐渐丧失，严重影响水稻生育后期有机物质的形成和积累，进而影响水稻产量的形成，是水稻高产潜力挖掘和发挥的主要限制因素之一[3]。叶片衰老作为一种程序性细胞死亡过程[4]，是植物的一种环境适应现象，当植物处于逆境时，有利于植物生存并正常繁殖后代，使种质得以延续，但叶片衰老可导致叶片光合能力降