SLE患者外周血CD4^＋CD25^＋调节性T细胞细胞因子分泌的改变.pdfVIP

匿 科堂象志2007年l2月第34卷第12其f · 685· · I临床论著· SLE患者外周血CD4 CD25 调节性T细胞细胞因子分泌 的改变 杨晓帆，张春兵，季晓辉 摘要：目的 研究活动性系统性红斑狼 (Sl E)患者外周血CD4 CD25 调节性T细胞白细胞介素(IL)一 10、转化生长L大J子(TGF)一p、干扰素(IFN)一^y和II 4分泌水平的改变。方法 免疫磁珠分离活动性SLE 患者和正常对照组CD4 CD25 调节性T细胞，流式细胞术(FCM)检测表达IL一10、IL4或IFN一^y的阳性 细胞百分率，体外培养两组CD4 CD25 渊节性T细胞，酶联免疫吸附法(ELISA)俭测培养细胞上清液 中IL一10、TGF-p和IFN一^y的水平。结果 在CD4 CD25一和CD4 CD25 T细胞中未发现分泌IL4的细 胞亚群；SI E患者CD4 CD25 T细胞中分泌lL一10的细胞亚群比例较正常人减少(P0．05)；C1M CD25和CD4 CD25 T细胞中均存 分泌IFN一^y的细胞亚群，且sl E患者自分泌IFN一^y的细胞亚群比 例增高(P0．05)。SLE患者c1)4 CD25 T细胞分泌IL—10和TGF—B较正常人均减少(P0．05)，自 分泌IFN一^y增多(P0．05)。结论 活动性SLE患者CD4 CD25 T细胞存在细胞因子分泌功能异常， 表现为TGF—p和lL一10分泌降低，IFN ^y产生增多。 关键词：系统性红斑狼疮； 细胞因子；凋节性T细胞 中图分类号：R593．241 文献标识码：A 文章编号：1004-2369(2007)12-0685-03 Cytokine secretion changes of peripheral CD4 CD25 regulatory T cells in patients with systemic lupus erythematosus YANG Xian—fan，ZHANG Chun—bing，JI Xiao—hui ．Department of Microbiology and Immu— nolog)r，Nanjing Medical University，Nanjing 210029，China． Corresponding author．E—mail：immune@njmu．edu．cn Abstract：Objective To investigate the secretion of interleukin(1L)一10，transforming growth factor(TGF)一B， interferon(IFN)一^y and II -4 of peripheral CD4 CD25 regulatory T cells in active systemic lupus erythemato— SHS(SLE)patients．Methods CD4 CD25 regulatory T cells were separated from peripheral blood samples of SLE patients’with magnetic activated cell sorting(MACS)．The positive proprtion of cells expressing I1 一 10，1l -4 and IFN一^y were measured with flow eytometry(FCM)．IL一10．TGF—p and IFN一^y seerected by CD4 CD25 ．r cells were measured with enzyme—linked immunosorbent assays(ELISA)．Results There were no IL一4 secreting subsets in CD4 CD25 and C D4 CD25 T cells．Compared with healthy controls．SLE patients had decreased proportion of IL一1 0 secrel【ing snbsets in CIM CD25 T cell