基于CCD 虚拟针孔探测的双轴共焦显微技术.PDF

36 5 Vol. 36 No . 5 2 0 1 0 9 OPTICAL T ECH NIQU E Sep. 2010 :2010) 基于CCD 虚拟针孔探测的双轴共焦显微技术 江琴, 邱丽荣, 赵维谦, 沙定国 ( , 100081) : , , CCD ( VPH) , , NA = i 0. 117, NA = 0. 106, = 45, ( FWH M) 2. 63m, ( NA= 0. 117) c FWH M 20 : ; CCD ; ; : TN247 : A Virtual pinhole dualaxes confocal microscopy based on CCD detector JIANG Qin, QIU Lirong, ZHAO Weiqian, SHA Dingguo ( School of Opticelect ronic, Beijing Institution of Technology , Beijing 100081, China) Abstract: In comparison w ith the conventional confocal microscopy , dualaxes confocal microscopy can use a low NA objective to achieve a high resolution, a large work distance and high S/ N, due to the special dualaxes structure. T he spat ial resolution of a dualaxes confocal m icroscopy wit h virtual pinhole CCD is analyzed in theory and its corresponding experiment al setup is established, and the axial response experiments are done using the established experimental setup. The full width at half a maxim ( FWH M) in the axial direct ion is 2. 63m achieved by using an illuminating lens of NA = i 0. 117, a collecting lens of NA = 0. 106 and = 45. And it is about 20 times higher than the axial FWH M of conventional c confocal microscopy with the same parameters. Key words : dualaxes confocal st ructure; CCD virtual pinhole;