细胞培养共同实验室简介.pdf

細胞培養 鄭旗志 細胞培養之醫學應用 Immuno-therapy Gene therapy Transfusion medicine Regenerative medicine Selection of antitumor medicine The characteristics of various cells 一.來源區分 ( 1).Cell lines (2).Primary cells 二.生長情形區分 1.貼附性 2.懸浮性 ( adhesion ) ( suspension ) A549 HL-60 細胞培養室之基本配備 1. 無菌操作台(Laminar Flow) 2. 5%CO2培養箱 3. 光學顯微鏡 4. 4℃冰箱 5. 37℃水浴槽 6. 離心機 7.液態氮桶 Cell culture incubator Laminar flow Microscope Refrigerator Centrifuger Water bath 細胞培養之基本概念 : 1 、培養細胞相關資訊之蒐集及閱讀 2 、選用適當之培養基 3 、無菌操作步驟 4 、解凍細胞及細胞之繼代培養 5 、冷凍細胞及細胞之長途運送 6 、細胞培養室之維護 Bioresources Collection and Research Center (BCRC) American Type Culture Collection (ATCC) Japanese Collection of Research Bioresources (JCBR) Culture medium RPMI1640 Dulbecco’s modified Eagle’s medium (DMEM) 培養基的成分 ● Inorganic salts ● Amino acid ●Vitamins ● Others *Glucose *Nucleotide *Pyruvate *Indicators-phenol red *pH buffer The role of sodium bicarbonate in culture medium (1). H2O+CO2 H2CO3 H+ + HCO3- + - (2). NaHCO3 Na + HCO3 List of amino acid Essential amino acid Nonessential amino acid Histidine Alanine Isoleucine Arginine Leucine Cysteine Lysine Glutamate Methionine