酶联免疫吸附分析法测定土壤中的毒死蜱-河南农业科学.pdf

DO I :10.15933/j .cnki .1004 -3268.2005.08.016 河南农业科学 1 1 2 1 1 韩丽君,钱传范 ,李文明, 江树人 , 王小如 (1., 100094;2., 450002 ) :采用自制多克隆抗体,考察了pH 值和甲醇含量对竞争反应的影响,并对反应体系进行了优 , 最终建立了土壤中毒死蜱的残留检测ELISA 方法。试验表明,毒死蜱对抗体竞争性结合的I50 为76.8 μg/L, 方法检出限为5.2 μg/L 。土壤样品经简单前处理后, 分别采用EL ISA 和GC 测定, 用ELISA 方法测得土壤中毒死蜱的添加回收率为 80.33%～83.36%,CV 为5.28%～9.83%, 最 低检出限为0 .005 mg/kg, 与G C 测定结果基本相当,符合农药残留分析的要求。 :酶联免疫吸附测定法;毒死蜱;土壤;残留检测 :S4 33 　　:A 　　:1004-3268 (2005 )08-0053-04 Determination o Chlorpy ri os in Soil by E LISA HAN Li-jun,Q IA N Chuan-an, LI Wen-ming,J IA NG Shu-ren,WAN G Xiao-ru (1.College o Science, China Ag ricultural U niversity, Beijing 100094, China; 2.College o Plant Protection, Henan Agricultural University,Zheng zhou 450002, China) Abstract:U sing t he sel -made poly-clonal antibody, t he a ect o pH and the met hanol content on the competitive react ion were investigated, and the reaction sy stem w as opt imized.A n indi rect enzy me- linked im munosorbent assay (EL ISA )or the determination o chlorpyri os residue in soil w as devel- oped.T he result s show ed t hat the chlorpyri os concent rat ion (I50 )that inhibit s 50%o antibody-anti- gen binding was 76.8 μg/L, the detection limit I20 w as 5.2 μg/L .Soil samples w ere detected w ith ELISA and G C a ter simple pre-procession .T he orti ied recovery o ELISA were 80.33%- 83.36%,CV w ere 5.28%-9 .83 %, the determinat ion limit was 0.005 mg/kg .The results o the ELISA w ere veri ied by GC met hod. Key words:EL ISA;Chlorpyri os;Soil;Pesticides residue test 　　, ,、。, :O , O --O -(3,5, 6--2 (EL ISA ) -)。, 、、、 , , 。、、 。 ,, , , M ancl is [1] [3] 2 ～4 ,