口腔鳞癌诱导型一氧化氮合酶mRNA的表达及其临床意义.pdfVIP

· 330· 肿瘤2008年4月第28卷第4期 Tumor Vo1．28，No．4，April，2008 DOI：10．3781／j．issn．1000-7431．2008．04．014 CiimealResearch,l 临畚 研究 口腔鳞癌诱导型一氧化氮合酶mRNA的表达及其临床意义 黄小梅，郑兰荣，顾 倩，邵金贵 (皖南医学院病理教研室，芜湖 241000) [摘要] 目的：探讨诱导型一氧化氮合酶(inducible nitric oxide synthase，iNOS)在口腔鳞癌(oral squamous cell carcinoma， OSCC)发生、发展中的作用及其与OSCC的临床病理特征之间的关系。方法：运用原位杂交技术检测1O例口腔正常黏膜、12 例单纯增生上皮、28例不典型增生上皮及32例鳞癌组织中iNOS mRNA的表达情况。结果：正常口腔黏膜组织缺乏iNOS mRNA表达；不典型增生组和鳞癌组中iNOS mRNA的表达较单纯增生组均显著增加(P0．001)；随着上皮不典型增生程度 的加重，iNOS mRNA表达显著增强(P0．05)；不典型增生组与鳞癌组之间iNOS mRNA的表达差异无统计学意义(P 0．05)。高、中、低分化的3种鳞癌组织中iNOS mRNA表达差异有统计学意义(P0．05)。口腔鳞癌患者Ⅲ、Ⅳ期组与有颈淋 巴结转移组iNOS mRNA表达分别明显高于I、Ⅱ期组与无颈淋巴结转移组(P0．05)。结论：iNOS mRNA可能在口腔鳞癌 的发生、发展中起重要作用；口腔鳞癌的组织分化程度、TNM分期及颈淋巴结转移与iNOS mRNA表达关系密切。iNOS的检 测可能对口腔鳞癌患者的预后判定和指导治疗具有参考意义。 [关键词] 口腔肿瘤；淋巴转移；原位杂交；诱导型一氧化氮合酶 [中图分类号] R739．85 [文献标志码] A [文章编号] 1000-7431(2008)04-0330-04 Expression of inducible nitric oxide synthase mRNA in oral squamous cell carcinoma and its clinical significance HUANG Xiao—mei。ZHENG Lan-rong，GU Qian，SHAO Jin-gui (Department of Pathology。Wannan Medical College，Wuhu 241000。China) [ABSTRACT] Objective：To study the role of mRNA expression of inducible nitric oxide synthase(iNOS)in the occurrence and development of oral squamous cell carcinoma(OSCC)and the relationship between the expressions of iNOS mRNA and the clinical pathologic characteristics of OSCC．Methods：In situ hybridization assay was used to detect iNOS mRNA in 10 cases of normal oral mu． cosa，12 cases of oral epithelial simple hyperplasia，28 cases of oral epithelial atypicall hyperplasia，and 32 cases of OSCC．Results： The expressions of iNOS mRNA was negative in the normal oral mucosa．The expression of iNOS mRNA in both atypical hyperplasia and OSCC groups were significantly higher than that in simple hyperplasia group(P0．001)．Statistical analysis showed a significant cor- relation between iNOS mRNA expression and the grade of atypic