壳聚糖包被磁性－荧光多功能复合粒子的研究 - 中山大学化学与化学 .doc

壳聚糖包被磁性－荧光多功能复合粒子的研究 chitosan -Coated Luminescent/Magnetic Silica Microspheres research 刘少君 [摘要] 集磁性和荧光于一体的复合型纳米粒子以其良好的应用前景备受关注。本文在磁性Fe3O4纳米颗粒表面包裹SiO2层，制备了Fe3O4@ SiO2复合粒子。复合粒子表面再连接阳离子聚合体羧甲基壳聚糖，通过静电作用，使带负电的量子点吸附于壳聚糖链上，最后将壳聚糖交联，形成稳定的荧光-磁性纳米复合粒子。通过红外、荧光光谱、扫描电镜、动态光散射等不同的分析测试手段对复合粒子性能进行了表征，结果表明成功制备了Fe3O4@SiO2@CMCH/CdTe复合粒子，该粒子是同时具有荧光和磁性的多功能纳米复合微粒，在靶向载药方面有着很广泛的应用。 关键词： 量子点; CdTe ； 羧甲基壳聚糖 ； 磁性纳米粒子 ； 荧光； 载药 [ Abstract ] Because of their great potential application, the multifunctional microspheres combining several advantages of fluorescence and magnetism had attracted great attention from scientists in many fields . In this article, silica-coated Fe3O4 particles were prepared at first, and then carboxymethylated chitosan (CMCH) was connected onto the surface of Fe3O4@SiO2 particles. By the electrostatic interaction between cationic CMCH and negatively charged CdTe quantum dots, CdTe nanoparticles were adsorbed on the chains of CMCH. Finally, Fe3O4@SiO2@CMCH/CdTe particles were obtained via the selective cross-linking of CMCH using glutaraldehyde. The as-prepared particles were characterized by transmission electron micrographs (TEM), fourier transform infrared (FTIR) spectroscopy, fluorescence spectroscopy and dynamic light scattering (DLS). It is indicated that Fe3O4@SiO2@CMCH/CdTe composite microspheres were prepared successfully. This microspheres are of fluorescence and magnetism, which is desired as carriers for diagnostics, target-oriented drug-delivery systems and so on. Key Words： quantum dot; CdTe；carboxymethylated chitosan ；magnetic nanoparticle ；fluorescence ；drug delivery纳米生物技术是国际生物技术领域的前沿和热点问题，在医药卫生领域有着广泛的应用和明确的产业化前景，特别是纳米药物载体、纳米生物传感器和成像技术以及微型智能化医疗器械等，将在疾病的诊断、治疗和卫生保健方面发挥重要作用。 柠檬酸三钠 分析纯　天津市耀华化学试剂有限公司 Te 　　　New Jersery USA 　 CdCl2 　　New Jersery USA 巯基丙酸（MPA） New Jersery USA NaHB　　国药集团化学试剂有限公司 NaOH　　广州化学试剂厂　 　 HCl　　　分析纯　广州化学试剂厂　　 FeCl2 ·4H２ O 　分析纯　广州化学试剂厂　　　 FeCl3 ·6H２ O 　分析纯　广州化学试厂　　 无水乙醇　　分析纯　广州化学试剂厂　　 乙酸