文献阅读-呼吸道感染类病毒探针诊断优化.pptVIP

Detecting respiratory viral RNA using expanded genetic alphabets and self-avoiding DNA 季志华 2015201188 北京化工大学 生研一班 BE NOT introduction Nucleic acid (NA)-targeted tests detect and quantify viral DNA and RNA (collectively xNA) to support epidemiological surveillance and, in individual patients, to guide therapy. They commonly use polymerase chain reaction (PCR) and reverse transcription PCR. advantages: 1, Sensitivity 2, rapid disadvantages: 1,expensive Multiplexing would allow their cost to be spread over multiple targets, but often only with lower sensitivity and accuracy, noise, false