图位克隆技术在农作物基因分离中的应用与评价 - 遗传.pdf

HEREDITAS (Beijing) 2010 9 , 32(9): 903 ―913 ISSN 0253-9772 综述 DOI: 10.3724/SP.J.1005.2010.00903 图位克隆技术在农作物基因分离中的应用与评价 1, 2 2 1 2 何俊平 , 阮松林 , 祝水金 , 马华升 1. , 310029; 2. , 310024 图位克隆(Map-based cloning)作为分离基因的有效方法, 已在小基因组作物的基因分离中得到了广泛应 用和发展, 而在具有大量重复 DNA 序列的大基因组作物中的应用仍存在挑战。基于图位克隆在基因分离中的 重要性, 文章就图位克隆技术的基本内容及发展做简要概述, 着重对图位克隆技术在大基因组作物中的应用进 行分析和评价, 同时对它今后可能的发展方向进行了讨论, 以期为大基因组作物基因的分离提供借鉴。 图位克隆; 基因分离; 大基因组 The application and evaluation of map-based gene isolation in crops 1, 2 2 1 2 HE Jun-Ping , RUAN Song-Lin , ZHU Shui-Jin , MA Hua-Sheng 1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; 2. Plant Molecular Biology and Proteomics, Institute of Biotechnology, Hangzhou Academy of Agricultural Science, Hangzhou 310024, China Abstract: As an efficient strategy for gene isolation, map-based cloning has been widely applied and well advanced in crops with small genomes. However, the application of this strategy is challenging in crops with large genomes that are rich in repetitive DNA. Because of the importance of map-based cloning, its basic contents and development are summarized. In particular, the application of this strategy is analyzed and evaluated in large genome crops, and the potential directions of its development are discussed. These help to promote genes cloning in large genome crops. Keywords: map-based cloning; gene isolation; large genome , : (Reverse genetics) , (Forward genetics)[1] , , , ,