超声辅助提取紫苏叶黄酮及其清除自由基作用研究.pdfVIP

西北林学院学报 2008，23(1)：158~161 Journal of Northwest Forestry University 超声辅助提取紫苏叶黄酮及其清除自由基作用研究 刘 宁， 仇农学 ， 田玉霞 (陕西师范大学食品工程系，陕西西安710062) 摘 要：紫苏是传统的药食同源植物，为进一步研究其生物活性成分，以乙醇溶液浸提，结合超声波 辅助方法，采用L。(3 )正交试验设计研究了紫苏叶黄酮的最佳提取工艺，并测定了其对羟自由基 (·OH)和1，1一二苯基一2一苦苯肼自由基(DPPH·)的清除效果。结果表明，黄酮最佳提取工艺条件 为：50 乙醇溶剂，料液比1：30，提取时间60 min，提取温度60℃，该条件下总提取率可达6．82 。 在一定范围内，提取物对·OH的清除效果与其浓度呈线性关系(R0—0．994 7)，Ics。为0．130 mg／ mL；对DPPH·有极强清除作用，其 。为0．032 mg／mL。试验表明紫苏叶含有丰富的黄酮类物 质，其具有体外抗氧化性，是一种有效的天然自由基清除剂，具有很大的开发利用前景。 关键词：紫苏叶；生物黄酮；超声波辅助提取；清除自由基 中图分类号：S 9．4 文献标识码：A 文章编号：1001—7461(2008)01—0158—04 Ultrasonic—aided Extraction of Flavonoids from Perilla frutescens(L．) Britt Leaves and Evaluation for Free—radical Scavenging LIU Ning，QIU Nong-xue ， TIAN Yu-xia (D 甜 ofFood Engineering，Shaanxi Normal University，Xi’an，Shaan~710062，Ch／na) Abstract：P la frutescens(L．)Britt was a kind of traditional nutriceutica1．In order to investigate its bioactivity components，the optimum conditions of extracting flavonoids from P．frutescens)leaves by u1一 trasonic—aided and ethanol extraction were studied by using L0(3 )orthogonal method．Meanwhile，free— radical scavenging capacities were investigated．The results showed that the optimal extraction condition were solvent 50 ethanol，the ratio of material to solvent was 1：30，and time of extraction 60 min，tem- perature 60C．In these conditions，the total extracting rate was as high as 6．82 ．Furthermore，a line relationship existed between scavenging of hydroxyl radical and the flavonoids in perilla leaves(R。一 0．994 7)，the Ics0(inhibitory concentration 50 )being 0．130 mg／mL．The flavonoids also showed very excellent inhibitory effects on 1，1-dipheny 1-2一picrylhydrazyl radical，and the was 0．032 mg／mL．The test indi— cated that there were abundant flavonoi