熏马肠中产生物胺氧化酶菌株的筛选与鉴定.pdf

现代食品科技 Modern Food Science and Technology 2014, Vol.30, No.5 熏马肠中生物胺氧化酶菌株的筛选与鉴定 马宇霞，卢士玲，李开雄，李蕊婷 （石河子大学食品学院，新疆石河子 832000） 摘要：为获得良好的产生物胺氧化酶的肉品发酵剂，本试验以新疆不同地区的44 种熏马肠样品为研究对象，对产生物胺氧化酶 菌进行筛选和鉴定，利用单层培养、双层显色的方法剔除产生物胺菌株，对分离后得到的菌株进行氧化酶试验、抑菌试验、耐盐和耐 亚硝酸盐试验。采用DGGE 剔除重复菌株，通过HPLC 对所筛选菌株氧化生物胺能力进行验证，最后对筛选出的菌株提取 DNA ，并 对其 16S rDNA 的V6-V8 区段进行PCR 扩增后测序。结果表明：本次试验共筛选出 6 株菌有氧化酶特性，耐盐能力在 6%以上，耐 亚硝酸盐能力在 0.15 g/kg 以上，对致病性大肠杆菌，单增李斯特菌，金黄色葡萄球菌三种致病菌有良好的抑制效果，经HPLC 验证 后氧化生物胺能力较强，分别为鼠李糖乳杆菌（Lactobacillus rhamnosus ），枯草芽孢杆菌（Bacillus subtilis ），腐生葡萄球菌（Staphylococcus saprophyticus ），木糖葡萄球菌（Staphylococcus xylosus ），戊糖片球菌（Pediococcus pentosaceus ），植物乳杆菌（Lactobacillus plantarum ）。 为发酵肉制品的工业化生产和安全性提供了良好的理论依据。 关键词：发酵肉制品；熏马肠；生物胺；DGGE；产生物胺氧化酶菌；高效液相色谱法 文章篇号：1673-9078(2014)5-49-55 Screening and Identification of Amine Oxidase Productive Strains from Smoked Horse Intestine MA Yu-xia, LU Shi-ling, LI Kai-xiong, LI Rui-ting (Food College, Shihezi University, Shihezi 832000, China) Abstract: In order to obtain good amine oxidase productive starters for meat product, amine oxidase productive strains were isolated from 44 samples in different areas of the smoked horse intestine. Productive amine bacteria were eliminated by double color method in monolayer culture. The oxidase test, bacteriostasis experiment and the resistance to salt and nitrite test were conducted for isolated strains. The same strains were eliminated by DGGE and the ability of oxidizing biogenic amine was verified by HPLC. Finally, the DNA was extracted from the screened strains, and V6-V8 segment of 16s rDNA were sequenced after PCR amplification. The results showed that six strains productive amine oxidase bacteria