淋巴细胞性J亚群禽白血病的诊断及其病理组织学观察.PDFVIP

安徽农业大学学报, 2016, 43(4): 547-550 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x028 网络出版时间：2016-7-12 16:52:59 [URL] /kcms/detail/34.1162.S1652.056.html 淋巴细胞性 J 亚群禽白血病的诊断及其病理组织学观察 * * 彭伟钊，杨志伟，汪 凯，祁克宗，王桂军，潘 玲 ，刘红梅 (安徽农业大学动物科技学院，合肥 230036) 摘 要：对 2 例疑似感染 J 亚群禽白血病病毒（J-Subtype Avian leukosis virus, ALV-J ）的病禽进行临床观察和 病理解剖，结果发现肝脏和脾脏均肿大，肝脏、心脏、肺脏、腺胃有肿瘤样结节；设计 1 对引物针对 ALV-J 的gp 85 基因，对 2 例病死鸡的肝脏组织进行 PCR 检测，结果 2 份组织样品均扩增出与预想结果一致的 924 bp 的ALV-J 特 异片段；取病死禽的心、肝、脾脏等组织制做石蜡切片，HE 染色，进行病理组织学观察，结果发现肝组织有大量 成淋巴细胞和淋巴样瘤细胞，呈灶状聚集；脾组织结构松散，白髓和红髓界限不清，脾小体消失，间隙有大量淋巴 样瘤细胞；心包脂肪、心肌间、心脏血管中大量淋巴样瘤细胞；腺胃肌层、腺胃乳头间隙有淋巴样瘤细胞，1 病例 腺胃乳头可见出血；肺泡隔及肺泡腔中有大量淋巴样瘤细胞填充等病理变化。试验结果证实，2 例土鸡均为淋巴细 胞性 J 亚群禽白血病。 关键词：淋巴细胞性 J 亚群禽白血病；诊断；PCR；病理观察 中图分类号：S852.65 文献标识码：A 文章编号：1672−352X (2016)04−0547−04 Diagnosis of lymphocytic J-subtype avian leukosis and its histopathologic observation PENG Weizhao, YANG Zhiwei, WANG Kai, QI Kezong, WANG Guijun, PAN Ling, LIU Hongmei (School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036) Abstract: Two cases of sick chickens, which were suspect to be infected with J-Subtype Avian leukosis virus (ALV-J), were diagnosed by clinical observation and pathological anatomy. The results indicated that swollen and tumor nodules were observed in liver, heart, spleen and lung. Based on the published gp85 sequence of ALV-J, a pair of PCR primers was designed to amplify the nucleic acids of ALV-J. The result found that a 924 bp spe- cific band that agrees with the expected size of ALV-J gp 85 was amplified from two liver tissues. The organs in diseased chickins, such as liver, heart, spleen, glandular stomach, and lung were ana