鸡源大肠杆菌浮游菌与生物被膜菌超广谱β-内酰胺酶的检测.pdfVIP

安徽农业大学学报，2008，35(2)：229—233 Journal of Anhui Agncultural University 鸡源大肠杆菌浮游菌与生物被膜菌超广谱13·内酰胺酶的检测 贾艳华，武博达，胡功政 ，李凤娟，晁利刚，朱理想 (河南农业大学牧医工程学院，郑州450002) 摘 要：采用平板培养法建立鸡源大肠杆菌生物被膜的体外模型，银染后经显微镜观察鉴定，并用双纸片协同 法检测浮游菌与生物被膜菌超广谱B一内酰胺酶的产生情况，同时用双光束紫外分光光度计测定超广谱p一内酰胺酶 的活性，为研究鸡源大肠杆菌生物被膜的耐药机制奠定方法学基础。结果表明，27株鸡源大肠杆菌均形成了生物 被膜，肉眼可以看到硅胶片上有黑色膜样物质形成，用银染法处理过的生物被膜菌在显微照相系统下可清楚地看 到生物被膜的结构；鸡源大肠杆菌生物被膜中超广谱 B一内酰胺酶的检出率(70．4％)高于浮游菌的检出率 (55．6％)，且生物被膜茵中超广谱B一内酰胺酶的酶活性((0．71±0．23)U·mg )高于浮游菌的酶活性((0．42± 0．12)U·mg )，前者是后者的1．73倍。 关键词：鸡源大肠杆菌；生物被膜；超广谱B一内酰胺酶；酶活性 中图分类号：$859．796 文献标识码：A 文章编号：1672—352X(2008)02—0229435 Detection of ESBLs produced in f0、 Escherichia coli biofilm JIA Yan—hua，WU B0一da，HU Gong—zheng，LI Feng-juan，CHAO Li—gang，ZHU Li—xiang (Technological College of Animal Husbandry and Veterinary Medicine，Henan Agricultural University，Zhengzhou 450002) Abstract：T0 lay the methodological basis on investigating the mechanism of drug resistance of fowl E．coli，the in vitro model of fowl E．col biofilm was established with the fiat．board method．and the biofilm was identified by the argentation and electron microscopy。then the ESBLs of the floatings and the biofilms were detected with the double．．disk diflusion method and the ESBLs activities were determined with the double beam UV spectrophotome— ter．The result revealed that all of the twenty．seven lowl E．col have formed biofilm．which was the black membrane type material seen by naked eye，and though micro—photography system the structure of biofilm can be seen clearly after the argentation treatment；the detection rate of fowl E．coli biofilm(70．4％)is higher than that of the corre— sponding floatings(55．6％)and the ESBLs activity ofE．coli biofilm((0．71±0．23)U-mg一 )is higher than that of the floatings((0．42±0．12)U·mg )． Key words：lowl E．coli；biofilm；ESBLs；enzymatic activities 随着头孢噻