硒化乳酸菌胞外多糖对小鼠腹腔巨噬细胞及肿瘤细胞内游离ca2+ 的影响.pdf

250 2014, Vol.35, No.01 食品科学 ※营养卫生 硒化乳酸菌胞外多糖对小鼠腹腔巨噬细胞及 肿瘤细胞内游离Ca2+ 的影响 * 刘 鹭，潘道东 ，丁 琳，曾小群 （宁波大学海洋学院食品系，浙江 宁波 315211） 摘  要：目的：研究硒化乳酸菌胞外多糖对小鼠腹腔巨噬细胞、SGC790 1胃癌细胞及海拉细胞（Hela cells ）内游离 Ca2+ 的影响。方法：分离制备小鼠腹腔巨噬细胞，利用Ca2+ 荧光探针Fluo-3/AM及激光共聚显微镜测定加药后10 min 细胞内Ca2+浓度的变化。结果：添加硒化多糖后，巨噬细胞内Ca2+浓度缓慢升高，而胃癌细胞与Hela细胞内Ca2+水平 均有不同程度的下降。结论：通过对乳酸菌胞外多糖进行硒化修饰可增强其刺激巨噬细胞的能力，因而发挥其免疫 功效。硒化多糖与肿瘤细胞表面受体结合后引起细胞内Ca2+ 内流的减少，提示硒化后的多糖可引起肿瘤细胞不同程 度的细胞凋亡。 关键词：胞外多糖；硒化；巨噬细胞；钙离子；肿瘤细胞 Effect of Selenium-Modified Exopolysaccharide from Lactococcus lactis subsp. lactis on [Ca2+] in i Mouse Macrophages and Cancer Cells * LIU Lu, PAN Dao-dong , DING Lin, ZENG Xiao-qun (Department of Food Science and Engineering , College of Marine, Ningbo University, Ningbo 315211, China) Abstract: Obj ective: The effect of Se-exopolysaccharide (EPS) from Lactococcus lactis subsp. lactis on intracellular free Ca2+ levels in mouse macrophages cells, gastric cancer cells SGC790 1 and Hela cells was studied. Methods: Cells labeled with Fluo-3/AM dye for 10 min were examined using laser confocal scanning microscope. Results: The intracellular Ca2+ level in mouse macrophages increased in the presence of Se-EPS. On the other hand, Se-EPS could decrease the intracellular Ca2+ levels both in gastric cancer cells SGC790 1 and Hela cells. Conclusion: Se-EPS can activate macrophages, which may be related to Ca2+ signal pathways. Se-rich EPS has gained the capacity to induceapoptosis in tumor cells. Key words: exopolysacc