121 cellulose acetate membrane electrophoresis 醋酸纤维素膜.ppt

* Genetic Engineering * 1.2.4 other types of electrophoresis two-dimensional electrophoresis * Genetic Engineering * 1.3 The application of electrophoresis techniques The electrophoresis techniques are used to separate and analyze the samples which have charge, especially protein and nucleic acids. According to the different purposes , we should choose different electrophoresis techniques. We have learned several kinds of electrophoresis techniques already. Would you please categorize them according to the different purposes? What is your opinion about the application of electrophoresis techniques in research and our life? Discussion in the class * * * * * * * * * * * * The electrophoresis techniques can be categorized according the different medium and analyzing subjects. The solid phase…and the liquid phase * * The electrophoresis techniques can be categorized according the different medium and analyzing subjects * * This technique was used to separate and analyze the protein usually, so here we would like to review some knowledge about the protein or peptides. The protein or peptides would have different charge in the different PH solution. So here is a question for you, what kinds of charge does the protein have when the PH higher than PI.The protein have negative charge migrate towards anode. * * This figure shows the cellulose acetate filter, * * The electrophoresis apparatus of cellulose acetate membrane * * There are two tanks full with electrolyte, * * There are two tanks full with electrolyte, the * * The nucleic acid have negative charge migrate towards anode. linear, superhelix, annular. It can migrate in the electric field within agarose gel that like a cribble, then the nucleic acid that differ in conformation can be separated by size. * * Typically, uncut plasmids will appear to migrate more rapidly than the same plasmid when linearized. Additionally, most preparations of uncut plasmid contain at least two topologically-different forms of