热老化对柞蚕丝蛋白二级结构的影响 - 《丝绸》杂志社.pdf

Vol.48 第48卷 2011年11月 第11期 No.11, Nov 2011 研究与技术 热老化对柞蚕丝蛋白二级结构的影响 李欢欢，傅吉全 (北京服装学院 材料科学与工程学院，北京 00029) 摘要：利用ATR-FTIR光谱技术和红外光谱去卷积拟合计算蛋白质二级结构百分含量，研究热老化对柞蚕丝纤维二级结构的影响，并 β 用XRD技术分析测定了老化后柞蚕丝的结晶度。结果表明：一定温度下，随着老化时间的延长，柞蚕丝 -sheet结构的相对百分含量下 α β 降，而 -helix结构和Random结构的相对百分含量上升；老化温度越高，柞蚕丝破坏的速度越快， -sheet结构的相对百分含量下降得 也越快，结晶度下降得也更加明显。 关键词：柞蚕丝；热老化；傅里叶红外光谱；二级结构 中图分类号：TS02.332 文献标志码：A 文章编号：00-7003(20)-0008-04 Effects of heat aging on the secondary structure of tussah silk protein LI Huan-huan, FU Ji-quan (School of Materials Science Engineering, Beijing Institute of Fashion Technology, Beijing 10029, China) Abstract: With the technology of ATR-FTIR spectroscopy and the approaches of Fourier self-deconvolution fitting to calculate quantitatively the percentage of the secondary structure of protein, the effects of heat aging on the secondary structure of tussah silk protein was investigated in this paper, combined with the analysis of the crystallinity of tussah silk by X-ray diffraction. The results showed that: under certain temperature, the β-sheet structure of silk percentage content will decrease, while the percentage content of α-helix structure and Random structure will increase, with the prolonged aging; the higher of temperature, the faster of decrease of β-sheet structure percentage content, and the more obvious decline in crystallinity will o