牦牛c1h21orf62 基因的克隆、 生物信息学及表达分析 - 广东农业科学.pdf

广东农业科学 2015 年第 5 期 98 牦牛C1H21orf62 基因的克隆、 生物信息学及表达分析 赵娟花 梁春年 裴 杰 褚 敏 吴晓云 良斌 佘平昌 阎 萍 中国农业科学院兰州畜牧与兽药研究所/甘肃省牦牛繁育工程重点实验室袁 甘肃 兰州 730050冤 要 应用 RT -PCR 方法克隆牦牛 C1H21orf62 基因并进行生物信息学分析袁利用实时荧光定量 PCR 方 法检测 C1H21orf62 基因在有角牦牛和无角牦牛角芽组织的mRNA 表达遥 结果表明袁C1H21orf62 基因编码区全 长 662 bp 袁编码 161 个氨基酸遥 C1H21orf62 是一种亲水性蛋白袁含有 13 个潜在的磷酸化位点袁但无跨膜域和信 号肽遥 C1H21orf62 基因 mRNA 在无角牦牛角芽组织的表达水平极显著高于有角牦牛遥 关键词 牦牛曰 无角曰 有角曰 C1H21orf62 曰 克隆 中图分类号 Q786 文献标识码 A 文章编号 1004-874X 2015冤05-0098-05 Cloning, bioinformatics and expression analysis of C1H21orf62 gene in yak ZHAO Juan-hua, LIANG Chun-nian, PEI J ie, CHU Min, WU Xiao-yun, ZHANG Liang-bin, SHE Ping-chang, YAN Ping (Lanzhou Institute of Husbandry and Pharmaceutical Sciences , Chinese A cademy of A gric ultural Sc ie nces/Key Laboratory of Y ak Breeding Engineering, Lanzhou 730050, China) A bstract: A coding region sequence of yak C1H21orf62 was amplified by RT -PCR. The physicochemical properties and domains in C1H21orf62 protein were predicted by bioinformatics methods. The expression levels of C1H21orf62 mRNA of horned and polled yaks were employed by Real -time PCR. The results showed that the coding region was 662 bp in length, encoding 161 amino acids. C1H21orf62 was a kind of hydrophilic protein w ith 13 potential phosphorylation sites, w ithout transmembrane domain and signal peptide. The expression of C1H21orf62 gene in polled yaks was higher than that in horned yaks significantly. Key words: yak; polled; horned; C1H21orf62; cloning 牦牛是生活于青藏高原等低氧尧高海拨地区的 通牦牛的野性增加袁易发生争斗袁从而给牦牛的饲