微脂粒包覆蚕丝蛋白之制备与功能性评估the manufacture - 农林学报.pdf

農林學報 64(1): 1-10 (2015) - 1 - Journal of Agriculture and Forestry, 64(1): 1-10 (2015) 微脂粒包覆蠶絲蛋白之製備與功能性評估 陳麗妃 1 陳麗舟 1 陳崇裕 2* 摘 要 本研究以不同脂質利用酒精注射法及薄膜水合 ／超音波震盪法，製備蠶絲蛋白微脂粒，並進行皮膚保濕及彈 力之功能性與配方安全性評估。實驗結果，不同製備方法以酒精注射法所得之微脂粒平均粒徑較小，約為 72~85 nm 之間；且包覆率較佳，約為 85~90%之間。在安定性方面，無論儲存於 4℃或室溫環境下，以薄膜/ 超音波震盪法製備 之微脂粒較為安定，顯示粒徑大小會影響其穩定性。在有效性方面，將不同方法製備的微脂粒配方（共 10 組）塗抺於 皮膚，分別測試保濕與彈力效果。以皮膚角質水分含量測試，各組配方在塗抹 120 min 後，顯示各組配方皆比控制組 佳，且具有保濕效果；連續塗抹於手臂內側四星期後，皮膚彈性效果也明顯增加。蠶絲蛋白微脂粒 10 組配方，經 10 人接受 48 小時過敏測試，並無過敏現象產生 。 蠶絲蛋白、微脂粒、皮膚保濕，皮膚有彈性。 The Manufacture and Effectiveness Evaluation of Liposome Incorporated with Silk Protein Li-Fei Chen 1 Li-Chou Chen 1 Chon-Yu Chen 2* ABSTRACT This study selected different lipids to prepare silk protein encapsulated liposomes by using ethanol injection method and thin film sonication/ethanol injection method. The prepared formulations also checked with safety test and skin hydration and elasticity. The experimental results showed smaller average particle size about 72~85 nm and better encapsulation rate about 85~90% by using ethanol injection method. For safety test, the sonication/ethanol injection method expressed better particle size stability for both 4℃ and room temperature. For efficacy test, ten different prepared methods and formulations were applied on skin to test skin hydration, elasticity and water content of stratum corneum. Every experimental samples treated 120 min showed better skin hydration than the control sample. After four weeks treatment with inner side of the arm, the skin elasticity also showed obviously improvement. These ten experimental samples also proceed with allergy test for ten people with 48 hours revealed no allergy positive results. Key Words: Silk Protein, Liposome, S