s 波段高功率微波辐射对原代培养乳鼠心肌细胞的影响.pdf

006 1 6 6 Chin J Radiol Med Prot, December 006, Vol 6, No.6 6 5 S 潘敏鸿 彭瑞云 王水明 高亚兵 马俊杰 徐新萍 王德文 徐天昊 董波 周红梅 S (HPM) 5~ 30 mWcm S HPM , + [ Ca ] 10~ i 30 mWcm S HPM 6 h, ( P 005 P 001) , + , ; 30 mWcm HPM [ Ca ] ( P 001) , i + 10~ 30 mWcm S HPM , [ Ca ] , i S HPM + ; ; ; [ Ca ] ; i Effect of Sband high power microwave radiation on primary cultured neonatal cardiomyocytes PAN Minhong , PENG Ruiyun, WANG Shuiming , et al. Institute of RadiationMedicine,A cademy of Military Medical Sciences, Beij ing 00850, China Corresponding author: PENG Ruiyun, Email: pengry@ nic . bmi. ac. cn Abstract Objective To investigate the effect of Sband high power microwave( HPM) radiation on primary cultured neonatal cardiomyocytes and explore its possible mechanism. Methods Primary cultured neonatal cardiomyocyteswere radiated with Sband HPM radiation with mean power density of 530 mWcm . Flow cytometry and atom force microscopy were used to determine the rates of apoptosis and necrosis, calcium ion concentration, and the structure of cell membrane. Results HPM radiation of 1030 mWcm increased the rates of apoptosis and necrosis( P 005 or P 001) and calcium ion concentration( P 001) , and caused membrane electroporation in cardiomyocytes. Conclusions HPM