拟南芥at3g57680基因编码的ctpa 蛋白对光系统ⅱ功能的影响.pdf

安徽农业大学学报, 2013, 40(3): 487-491 Journal of Anhui Agricultural University 网络出版时间：2013-4-26 15:37:38 [URL] /kcms/detail/34.1162.S1537.028.html 拟南芥at3g57680 基因编码的CtpA 蛋白对光系统Ⅱ功能的影响 1,2 1 1 金美芳 ，高 茜 ，张珠珠 (1. 兰州大学生命科学学院，兰州 730000 ；2. 福建师范大学福清分校生物与化学工程系，福清 350300) 摘 要：采用电镜技术、Northern 印迹技术、蓝绿温和胶电泳、SDS电泳和蛋白免疫印迹等方法对拟南 芥 at3g57680 基因缺失的突变体植株研究。结果表明：（1 ）突变体植株和野生型植株的叶绿体都呈扁球状，叶绿体 的大小基本一样，叶绿体内部基质，基粒均匀分布，基粒类囊体垛叠层数相近；（2 ）psbA 、psbB 、psbC 和psbO 基 因 mRNA 水平没有变化；（3 ）类囊体膜蛋白复合物的组成、表达量以及蛋白复合物各亚单位的组成和含量也是基 本一致，且突变体与野生型植株中 D1 蛋白的表达量也保持一致。说明在正常生长环境中，拟南芥 at3g57680 基因 的缺失对叶绿体显微结构、D1 蛋白的成熟加工以及 PS Ⅱ蛋白组成等没有影响，即at3g57680 基因编码的类 CtpA 蛋白对 PS Ⅱ功能没有影响。 关键词：拟南芥；CtpA；D1 蛋白；光系统Ⅱ 中图分类号：Q945 文献标识码：A 文章编号：1672−352X (2013)03−0487−05 Effect of CtpA protein encoded by at3g57680 gene on the function of photosystem II in Arabidopsis thaliana JIN Mei-fang 1, 2 , GAO Qian 1, ZHANG Zhu-zhu1 (1. College of Life Sciences, Lanzhou University, Lanzhou 730000； 2. Department of Biology and Chemical Engineering, Fuqing Branch of Fujian Normal University, Fuqing 350300) Abstract: Electron microscopy technique, Northern blot, Blue native gel, SDSgel and Western blot technique were used to analyse at3g57680 gene knocked mutant and wild plant of Arabidopsis thaliana . The results were as the follows: (1) The chloroplast in mutant plants and wild type plants were all in flat shape and had similar size; the granas and matrixs of chloroplast were uniformly distributed; the number of granum-thylakoids staek were similar in the mutant plants and the wild type plants. (2) The transcript levels of psbA psbB, psbC and psbO were the same in the mutant and wild type plants. (