rhizobium sp n613胞外多糖的抗氧化活性.doc

Rhizobium sp. N613胞外多糖的抗氧化活性 陈艳丽 任盛 魏国琴 吕利华 赵良启* (山西大学化学生物学与分子工程教育部重点实验室 山西 太原 030006) 摘 要: 以铁氰化钾和铁离子为检测系统测定了Rhizobium sp. N613胞外多糖(REPS)对氧化物的还原力; H2O2和Fe2+为羟自由基生成系统检测了REPS对羟自由基的清除作用; 邻苯三酚自氧化法检测了REPS对超氧阴离子的清除作用; 并体外检测了REPS对H2O2引起的氧化溶血现象的抑制作用及对小鼠肝组织脂质过氧化损伤的保护作用。结果显示, REPS对氧化物具有明显的还原力, 其对羟自由基的清除作用达到35.46%(多糖浓度为2 mg/mL), 对超氧阴离子的清除作用达到36.84% (多糖浓度为0.78 mg/mL), 对H2O2引起的氧化溶血现象的抑制作用达到43.84% (多糖浓度为1.14 mg/mL), 对小鼠肝组织脂质过氧化的保护作用达到34.46% (多糖浓度为1.14 mg/mL), 表明REPS具有良好的抗氧化作用, 有着重要的应用价值。锦鸡儿根瘤菌胞外多糖, 活性氧自由基, 脂质过氧化, 抗氧化活性Antioxidant Activity of Exopolysaccharide from Rhizobium sp. N613 CHEN Yan-Li REN Sheng WEI Guo-Qin LV Li-Hua ZHAO Liang-Qi* (Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, Shanxi 030006, China) Abstract: We investigated the reducing capacity of Caragana rhizobium exopolysaccharides (REPS) by the system of potassium ferricyanide and ferric chloride, determined the hydroxyl radical scavenging capacity of REPS by H2O2/Fe2+ system, measured the Superoxide radical scavenging capacity of REPS by the pyrogallol system, and we also determined its protective effects on H2O2 in hemplysis of erythrocytes and lipid per-oxidation of liver homogenate in rat. The results indicated that REPS has reductive capacity to oxides, the hydroxyl radical scavenging capacity of REPS was 35.46% (at the concentration of 2 mg/mL), the Superoxide radical scavenging capacity of REPS was 36.84% (at the concentration of 0.78 mg/mL), the protective effects of REPS on H2O2 induced hemolysis of rat erythrocytes was 43.84% (at the concentration of 1.14 mg/mL), and the protective effects on lipid per-oxidation of liver homogenate in rat was 34.46% (at the concentration of 1.14 mg/mL). Keywords: Exopolysaccharide from Rhizobium sp. N613, Reactive oxygen species, Lipid per-oxidation, Antioxidant activity 众所周知, 氧化还原反应是生物体新陈代谢的一个重要组成部分, 机体在生理代谢过程中自由基产生与清除一般处于动态平衡状态[1]。一旦这种动态平衡被破坏, 导致自由基超过机体耐受限度, 就会造成组织器官损伤和许多疾病发生。现代医学研究表明, 心脑