通络救脑注射液对aβ致脑微血管内皮细胞损伤的保护作用.docVIP

通络救脑注射液对Aβ致脑微血管内皮细胞损伤的保护作用 高爱社1，杜 欢2，范英昌2，王榭延1（1. 河南中医药大学, 河南 郑州 450008；天津中医药大学，天津300193） 摘要：目的 探讨通络救脑注射液及其有效成分对脑微血管内皮细胞的保护作用及机制。方法 建立；β淀粉样蛋白Aβ）致脑微血管内皮细胞 (BMECs)损伤模型，用通络救脑注射液及其有效单体成分干预，采用CCK-8染色法测定细胞活性，检测条件培养液中乳酸脱氢酶漏出量，免疫蛋白印迹法检测脑微血管内皮细胞高度糖基化终产物（RAGE）的表达。结果 通络救脑注射液可显著提高Aβ损伤的脑微血管内皮细胞活性，减少细胞损伤后LDH的漏出和脑微血管内皮细胞RAGE的表达，与模型组相比， 差异具有统计学意义（P0.01），通络救脑注射液作用明显优于两个单体成分。结论 通络救脑注射液可提高Aβ损伤的脑微血管内皮细胞的活性，对其具有保护作用，其机制可能与减少血管内皮细胞RAGE 的表达有关，通络救脑注射液的作用优于其单体成分。 关键词：通络救脑注射液；脑微血管内皮细胞；高度糖基化终产物；β淀粉样蛋白 中图分类号：R285.5； 文献标志码：A 文章编号：1003-9783(2016) 02- doi: 10.3969/j.issn.1003-9783.2016.02. Protective Effects of Tong Luo Jiu Nao Injection and Its Active Ingredients on Brain Microvascular Endothlial Cells injured byβ-amyloid GaoAi-she1 DuHuan2 FanYing-chang2 WangXie-yan1 College of Basic Medicine,Henan Universty of Chinese Medicine 2. College of Basic Medicine,Tinjin Universty of Chinese Medicine ［ Abstract ］ Objective To evaluate the protective effects of Tong Luo Jiu Nao（ TLJN）Injection，and its active ingredients(including Geniposde and GinsenosideRg1), on human brain microvascular endothelial cells (BMECs) injured by β-amyloid，via observing their effects on the activies of BMECs and the expression of the receptor for advanced glycation endproducts (RAGEs) of brain endothelial cells. Methods Human brain microvascular endothelial cells were pre-incubated for 2h by TLJN and Geniposde and GinsenosideRg1， then injured by β-amyloid. At different intervals，Cell Counting Kit-8 was employed to ossay the activities of BMECs, the damage elevel of BMECs was evaluated using lactate dehydrogenase (LDH) leaka gerate, and the expression levels of Receptor for advancedglycation endproducts( RAGE )by Western blot. Results TLJN Injection could significantly improve the activities of Human brain microvascular endothelial cells injured by Aβ, and decreased LDH leaka gerate，and down-regulate the expression levels of RAGE . Two active ingredients showed different effects at different int